ANTIGENIC RELATIONSHIP BETWEEN HUMAN CORONAVIRUS STRAIN OC-43 AND HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS-STRAIN 67N OF SWINE - ANTIBODY-RESPONSES IN HUMAN AND ANIMAL SERA
ANTIGENIC RELATIONSHIP BETWEEN HUMAN CORONAVIRUS STRAIN OC-43 AND HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS-STRAIN 67N OF SWINE - ANTIBODY-RESPONSES IN HUMAN AND ANIMAL SERA
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DOI：
10.1093/infdis/135.2.201
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发表时间：
1977-01-01
影响因子：
6.4
通讯作者：
HARRISON, AK
中科院分区：
文献类型：
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作者：
KAYE, HS;YARBROUGH, WB;HARRISON, AK
猪血凝性脑脊髓炎病毒(HEV)适应乳鼠脑内生长。用阴性染色和薄片技术制备的HEV感染乳鼠脑的EM具有典型的与冠状病毒科其他成员相同的形态特征。HEV对哺乳小鼠脑的适应通过使用通用宿主试剂和相容的动物系统促进了血清学测试。通过血凝抑制、补体结合和中和试验，证实了特异性免疫和高免动物血清中人冠状病毒OC43和HEV之间的抗原性关系。血清转换为OC43抗原的儿童和成人对HEV抗原抗体效价的诊断升高。血清转换为人类冠状病毒229E和B814的个体显示出对HEV的抗体，但没有诊断效价上升。具有HEV抗体滴度的猪具有较低的或没有检测到冠状病毒OC43抗体滴度。虽然OC43抗体的流行率和几何平均滴度均高于HEV抗体滴度，但与猪有过接触或没有接触过猪的人群对冠状病毒OC43和HEV的抗体反应有显著差异。人类中的HEV抗体可能代表了对冠状病毒OC43感染的异种反应.然而，不能排除对未知或未表征的冠状病毒株的异型反应。
Hemagglutinating encephalomyelitis virus of swine (HEV) was adapted to growth in suckling mouse brain. EM of HEV-infected suckling mouse brain, prepared by negative staining and thin-section techniques, exhibited typical morphological characteristics shared with other members of the Coronaviridae. The adaptation of HEV to suckling mouse brain facilitated serologic testing by the use of common host reagents and compatible animal systems. With hemagglutination inhibition, complement-fixation, and neutralization tests an antigenic relationship was demonstrated between human coronavirus OC 43 and HEV in specific immune and hyperimmune animal sera. Children and adults with seroconversion to OC 43 antigen had diagnostic rises in titer of antibody to HEV antigens. Individuals with seroconversion to human coronaviruses 229E and B814 demonstrated antibody to HEV but not diagnostic rises in titer. Swine with titers of antibody to HEV had lower or no detectable titers of antibody coronavirus OC 43. Although the prevalence and geometric mean titer of antibody to OC 43 were higher than the titer of antibody to HEV in every group of normal humans tested, significant differences in antibody response to coronavirus OC 43 and HEV were seen between populations that did or did not have possible contact with swine. Antibody to HEV in humans probably represented a heterologous response to infection with coronavirus OC 43. However, a heterotypic response to unknown or uncharacterized strains of coronavirus cannot be excluded.