New class of transcription factors controls flagellar assembly by recruiting RNA polymerase II in Chlamydomonas (Publication with Expression of Concern. See vol. 118, 2021)
New class of transcription factors controls flagellar assembly by recruiting RNA polymerase II in Chlamydomonas (Publication with Expression of Concern. See vol. 118, 2021)
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新型转录因子通过招募衣藻中的 RNA 聚合酶 II 来控制鞭毛组装
DOI：
10.1073/pnas.1719206115
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发表时间：
2018-04-24
影响因子：
11.1
通讯作者：
Hu, Zhangfeng
中科院分区：
文献类型：
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作者：
Li, Lili;Tian, Guangmei;Hu, Zhangfeng
意义鞭毛基因的转录调控控制鞭毛组装的第一步。在这项研究中，我们发现XAP5是一种功能未知的保守蛋白质，它定义了一类转录因子，用于调控参与鞭毛组装的基因的转录。鞭毛再生过程中X染色体相关蛋白5(XAP5)的磷酸化跟踪鞭毛长度。值得注意的是，RNA聚合酶II(POL II)转录激活机制的招募依赖于XAP5的活性。我们的数据表明，XAP5作为一个转录因子，通过招募RNA Pol II来调节鞭毛基因的转录。我们的结果加深了我们对XAP5家族的生化功能和鞭毛组装的转录调控的理解。细胞已经开发出支持鞭毛组装和维护的调节机制，包括鞭毛基因的转录调节，这是制造鞭毛的第一步。虽然衣藻的鞭毛组装需要对鞭毛基因表达的转录调控，但目前还没有发现调控鞭毛基因转录的转录因子。我们报道了X染色体相关蛋白5(XAP5)作为一个转录因子来调节衣藻的鞭毛组装。虽然XAP5蛋白在进化上在不同的生物体中是保守的，并在不同的生物过程中发挥着重要的作用，但对这个重要蛋白质家族的任何成员的生化功能都知之甚少。我们的数据表明，XAP5的丢失会导致鞭毛组装的缺陷。XAP5的翻译后修饰在鞭毛组装过程中跟踪鞭毛长度，表明细胞具有调节XAP5修饰的反馈系统。值得注意的是，XAP5通过直接与包含CTGGGGGTG-core的基序结合来调节鞭毛基因的表达。此外，RNA聚合酶II(POL II)转录激活机制的招募依赖于XAP5的活性。我们的数据表明，通过Pol II的招募，XAP5定义了一类转录调控纤毛基因的转录因子。这项工作提供了对XAP5家族的生化功能和鞭毛组装的基础生物学的见解，加深了我们对鞭毛的信号和功能的理解。
Significance Transcriptional regulation of flagellar genes controls an initial step in flagellar assembly. In this study, we show that XAP5, a conserved protein of unknown function, defines a class of transcription factor for transcriptional regulation of genes involved in flagellar assembly. Phosphorylation of X chromosome-associated protein 5 (XAP5) during flagellar regeneration tracks flagellar length. Remarkably, recruitment of RNA polymerase II (Pol II) machinery for transcriptional activation depends on the activities of XAP5. Our data demonstrate that XAP5 functions as a transcription factor for transcriptional regulation of flagellar genes through recruitment of RNA Pol II. Our results enhance our understanding of the biochemical function of the XAP5 family and the transcriptional regulation of flagellar assembly. Cells have developed regulatory mechanisms that underlie flagellar assembly and maintenance, including the transcriptional regulation of flagellar genes, an initial step for making flagella. Although transcriptional regulation of flagellar gene expression is required for flagellar assembly in Chlamydomonas, no transcription factor that regulates the transcription of flagellar genes has been identified. We report that X chromosome-associated protein 5 (XAP5) acts as a transcription factor to regulate flagellar assembly in Chlamydomonas. While XAP5 proteins are evolutionarily conserved across diverse organisms and play vital roles in diverse biological processes, nothing is known about the biochemical function of any member of this important protein family. Our data show that loss of XAP5 leads to defects in flagellar assembly. Posttranslational modifications of XAP5 track flagellar length during flagellar assembly, suggesting that cells possess a feedback system that modulates modifications to XAP5. Notably, XAP5 regulates flagellar gene expression via directly binding to a motif containing a CTGGGGTG-core. Furthermore, recruitment of RNA polymerase II (Pol II) machinery for transcriptional activation depends on the activities of XAP5. Our data demonstrate that, through recruitment of Pol II, XAP5 defines a class of transcription factors for transcriptional regulation of ciliary genes. This work provides insights into the biochemical function of the XAP5 family and the fundamental biology of the flagellar assembly, which enhance our understanding of the signaling and functions of flagella.