Direct encapsulation of proteins into calcium silicate microparticles by water/oil/water interfacial reaction method and their responsive release behaviors
Direct encapsulation of proteins into calcium silicate microparticles by water/oil/water interfacial reaction method and their responsive release behaviors
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DOI：
10.1016/j.msec.2012.07.030
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发表时间：
2012-12-01
影响因子：
7.9
通讯作者：
Kubota, Takayuki
中科院分区：
文献类型：
--
作者：
Fujiwara, Masahiro;Shiokawa, Kumi;Kubota, Takayuki
采用水/油/水乳状液（W/O/W乳状液）界面反应法，研究了溶菌酶、牛血清白蛋白（BSA）和卵清蛋白等蛋白质在硅酸钙微球中的直接乳化作用，为制备包埋生物大分子的二氧化硅和碳酸钙微胶囊提供了有效的方法。这些蛋白质添加到硅酸钠溶液的W/O/W乳液成功地封装到微粒中，这是确定的漫反射紫外（UV）和红外光谱。蛋白质的包封效率取决于蛋白质的分子量，这在蛋白质包封到二氧化硅和碳酸钙微胶囊中也是可观察到的。通过用去离子水简单洗涤没有排出包封的蛋白质，表明蛋白质安全包装到硅酸钙基质中。乙二胺-N，N，N '，N'-四乙酸四钠盐（EDTA-4 Na）有效地从微粒中提取钙离子以作为蛋白质释放的触发剂，表明钙离子的消除启动了包封的蛋白质的释放。钙和钠离子之间的离子交换也实现了蛋白质释放到水溶液中。当氯化钠浓度小于1 M时，溶菌酶和BSA等蛋白质的释放速率增加。在稀释的溶液中，氯化钾比氯化钠更能加速蛋白质的释放。这些特征释放行为必须应用于响应于体液中碱性离子浓度的药物递送系统。(C)2012爱思唯尔有限公司版权所有。
The direct encapsulations of proteins such as lysozyme, bovine serum albumin (BSA) and ovalbumin into calcium silicate microparticles were examined by interfacial reaction method using water/oil/water emulsion (W/O/W emulsion), which was effective for the preparations of silica and calcium carbonate microcapsules encapsulating biomacromolecules. Those proteins added to the sodium silicate solution of the W/O/W emulsion were successfully encapsulated into the microparticles, which were ascertained by diffuse reflectance ultraviolet (UV) and infrared spectra. The encapsulation efficiency of the proteins depended on the molecular weight of proteins, which were also observable in the protein encapsulations into silica and calcium carbonate microcapsules. No discharge of encapsulated proteins by simple washing with deionized water indicated the secure packing of proteins into calcium silicate matrix. Ethylenediamine-N,N,N',N'-tetraacetic acid, tetrasodium salt (EDTA-4Na) effectively extracted calcium ions from the microparticles to serve as a trigger for the release of proteins, indicating that the elimination of calcium ion initiated the discharge of encapsulated proteins. The ion exchange between calcium and sodium ions also achieved the release of proteins into aqueous solutions. The release rate of proteins such as lysozyme and BSA increased with the concentration of sodium chloride less than 1 M. In diluted solutions, potassium chloride accelerated the protein release more than sodium chloride. These characteristic release behaviors must be applied to drug delivery systems that respond to the concentration of alkaline ions in body fluid. (C) 2012 Elsevier B.V. All rights reserved.