Expression and nucleotide sequence analysis of the Legionella pneumophila recA gene.
Expression and nucleotide sequence analysis of the Legionella pneumophila recA gene.
复制标题
嗜肺军团菌recA基因的表达和核苷酸序列分析。
DOI：
10.1111/j.1574-6968.1990.tb13983.x
复制
发表时间：
1990
影响因子：
2.1
通讯作者：
Dreyfus,LA
中科院分区：
文献类型：
--
作者：
Zhao,X;Dreyfus,LA
The nucleotide sequence of theL. pneumophila recAgene was determined. The coding region was 1044 nucleotides (348 codons), specifying a 37 934 Da protein. Preceding therecAgene was a tandem set of transcription regulatory sequences and putative LexA binding sites. When expressed inE. coli, the clonedrecAgene yielded two proteins with molecular weights of approximately 38 000 and 35 500 Da. The larger of these two proteins shared 70.4% and 74.6% identity with theE. coliandPseudomonas aeruginosaRecA proteins, respectively. The 35 500 DarecAencoded protein was presumed to be the product of translation from Met26which was preccded by an alternate ribosomal binding site.
