Electron microscopy of mouse preimplantation embryos and isolated blastomeres immobilized on coverslips
Electron microscopy of mouse preimplantation embryos and isolated blastomeres immobilized on coverslips
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电子显微镜观察固定在盖玻片上的小鼠植入前胚胎和分离的卵裂球
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
M. Yamagata
中科院分区：
文献类型：
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作者：
L. Wiley;Kathy K. Takaki;M. Yamagata
在这份报告中，我们描述了一种新的方法，固定完整的小鼠植入前胚胎及其分离的卵裂球在一个平坦的基板上，使他们可能会被处理enabling进行电子显微镜。胚胎可以被布置成使得它们将是共面的并且足够紧密地分组在一起以被包括在同一截面平面内。基质由涂有成纤维细胞层或纤连蛋白的Thermonex塑料盖玻片组成。植物凝集素phytohemaggiutinin（PHA）被用作将胚胎粘附到盖玻片上的配体。该方法与其他配体和各种免疫细胞化学程序兼容，可用于活的或固定的胚胎。
In this report we describe a new method of immobilizing intact mouse preimplantation embryos and their dissociated blastomeres on a flat substrate so that they may be processed en masse for electron microscopy. The embryos can be arranged so that they will be coplanar and grouped together closely enough to be included within the same plane of section. The substrate consists of Thermonex plastic coverslips that have been coated with a layer of fibroblasts or with fibronectin. The plant lectin phytohemaggiutinin (PHA) is used as a ligand to adhere the embryos onto the coverslips. This method is compatible with other ligands and with a variety of immunocytochemical procedures and can be used with live or fixed embryos.