Phylogenetic and structural relationships of the PR5 gene family reveal an ancient multigene family conserved in plants and select animal taxa
Phylogenetic and structural relationships of the PR5 gene family reveal an ancient multigene family conserved in plants and select animal taxa
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DOI：
10.1007/s00239-005-0053-z
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发表时间：
2006-07-01
影响因子：
3.9
通讯作者：
Weathersbee, A. A., III
中科院分区：
文献类型：
--
作者：
Shatters, Robert G., Jr.;Boykin, Laura M.;Weathersbee, A. A., III
发病机制相关第 5 族 (PR5) 植物蛋白包括奇异果甜蛋白、渗透压蛋白和相关蛋白，其中许多具有抗菌活性。最近在线虫和昆虫中发现的 PR5 样 (PR5-L) 序列引发了关于它们的进化关系的问题。利用完整的植物基因组数据和多个昆虫 PR5-L 序列的发现，对植物和动物之间的系统发育进行了比较。所有 PR5/PR5-L 蛋白序列均从两个主要被子植物类群（真双子叶植物（拟南芥）和单子叶植物（稻））和秀丽隐杆线虫（秀丽隐杆线虫和布里格斯线虫）的基因组数据中挖掘。昆虫 PR5-L 序列是从 EST 数据库和 GenBank 提交的四种昆虫目中挖掘的：鞘翅目（Diaprepes abbreviatus 和 Biphyllus lunatus）、直翅目（Schistocerca gregaria）、膜翅目（Lysiphlebus testaceipes）和半翅目（Toxoptera citricida）。简约性和贝叶斯系统发育分析表明，PR5 家族在植物中是并系的，可能源自单子叶植物和真双子叶植物共同祖先的 10 个基因。单子叶植物和真双子叶植物进化分化后，PR5基因在10个进化枝中不对称增加。昆虫和线虫包含多个序列（线虫中有 7 个 PR5-L，某些昆虫至少有 3 个），全部与同一植物进化枝相关，线虫和昆虫序列分为两个进化枝。蛋白质结构同源性模型显示动物和植物 PR5/PR5-L 之间具有很强的相似性，仅在表面暴露的环中存在差异。 PR5/PR5-L 之间的序列和结构保守性表明，它们在其所属的不同生物体的进化分化过程中发挥着重要且保守的作用。
Pathogenesis-related group 5 (PR5) plant proteins include thaumatin, osmotin, and related proteins, many of which have antimicrobial activity. The recent discovery of PR5-like (PR5-L) sequences in nematodes and insects raises questions about their evolutionary relationships. Using complete plant genome data and discovery of multiple insect PR5-L sequences, phylogenetic comparisons among plants and animals were performed. All PR5/PR5-L protein sequences were mined from genome data of a member of each of two main angiosperm groups-the eudicots (Arabidoposis thaliana) and the monocots (Oryza sativa)-and from the Caenorhabditis nematode (C. elegans and C. briggsase). Insect PR5-L sequences were mined from EST databases and GenBank submissions from four insect orders: Coleoptera (Diaprepes abbreviatus and Biphyllus lunatus), Orthoptera (Schistocerca gregaria), Hymenoptera (Lysiphlebus testaceipes), and Hemiptera (Toxoptera citricida). Parsimony and Bayesian phylogenetic analyses showed that the PR5 family is paraphyletic in plants, likely arising from 10 genes in a common ancestor to monocots and eudicots. After evolutionary divergence of monocots and eudicots, PR5 genes increased asymmetrically among the 10 clades. Insects and nematodes contain multiple sequences (seven PR5-Ls in nematodes and at least three in some insects) all related to the same plant clade, with nematode and insect sequences separating as two clades. Protein structural homology modeling showed strong similarity among animal and plant PR5/PR5-Ls, with divergence only in surface-exposed loops. Sequence and structural conservation among PR5/PR5-Ls suggests an important and conserved role throughout the evolutionary divergence of the diverse organisms from which they reside.