Chromosomal mapping of Brassica oleracea based on ESTs from Arabidopsis thaliana:: complexity of the comparative map
Chromosomal mapping of Brassica oleracea based on ESTs from Arabidopsis thaliana:: complexity of the comparative map
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DOI：
10.1007/s00438-002-0782-2
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发表时间：
2003-02-01
影响因子：
3.1
通讯作者：
Sadowski, J
中科院分区：
文献类型：
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作者：
Babula, D;Kaczmarek, M;Sadowski, J
利用拟南芥基因组测序项目中的表达序列标签（EST）构建了甘蓝的RFLP图谱。在110 A。通过对拟南芥ESTs的分析，共筛选出95个RFLP探针。在现有的B遗传图谱中，总共添加了212个新位点，对应于95个EST。甘蓝。丰富的地图涵盖了所有9个基本连锁群，并证实了染色体的B。oleracea和A.在线性组织中，拟南芥是相似的。然而，B的染色体之间的不同水平的序列保守性。oleracea和A.在基因组的不同区域检测到了拟南芥。在两个基因组中包含整个染色体臂的长保守区域被确定;这些可能是由血统共享的。另一方面，在许多染色体区域观察到广泛的重排，产生A.在芸苔属的基因组中存在类似拟南芥的片段。在A.在构建B的比较图时考虑了thaliana。oleracea和A. thaliana.
Expressed sequence tags (ESTs) from the Arabidopsis thaliana sequencing project were used to construct a genetic RFLP map for Brassica oleracea. Of the 110 A. thaliana ESTs tested, 95 were found to be informative RFLP probes in map construction. In total, 212 new loci corresponding to the 95 ESTs were added to the existing genetic map of B. oleracea. The enriched map covers all nine basic linkage groups and confirms that the chromosomes of B. oleracea and A. thaliana are similar in linear organization. However, varying levels of sequence conservation between the chromosomes of B. oleracea and A. thaliana were detected in different regions of the genomes. Long conserved regions encompassing entire chromosome arms in both genomes were identified; these are probably shared by descent. On the other hand, extensive rearrangements were observed in numerous chromosome regions, producing a mosaic of A. thaliana-like segments in the genome of Brassica. The presence of extensive chromosome duplication in A. thaliana was taken into consideration in the construction of the comparative maps of B. oleracea and A. thaliana.