Effects of Pharmacological Agents on Human Amniotic Fluid-Derived Stem Cells in Culture
Effects of Pharmacological Agents on Human Amniotic Fluid-Derived Stem Cells in Culture
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DOI：
10.1089/scd.2016.0141
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发表时间：
2016-10-15
影响因子：
4
通讯作者：
Young, Bruce K.
中科院分区：
文献类型：
--
作者：
Dziadosz, Margaret;Chan, Michael;Young, Bruce K.
羊水含有多能细胞，可能是临床使用的干细胞来源。羊水细胞（AFC）由胎儿细胞的异质群体组成，可以在妊娠期间回收而没有伦理问题，因为它是标准的临床程序。在与地塞米松、丙戊酸和硫酸镁一起培养后，进行增殖、多能标记物表达、分化和基因表达的研究。已知药物对人类胎儿的影响与培养中人类AFC的变化之间存在相关性，以及以前未描述的神经和软骨诱导的观察结果。基因表达谱证实了这些观察结果。AFC文化可能提供了一种新的方法来评估药物在临床使用前在怀孕。
Amniotic fluid contains multipotent cells and could be a source of stem cells for clinical use. Amniotic fluid cells (AFCs) are made up of a heterogeneous population of fetal cells that can be retrieved during pregnancy without ethical concerns as it is a standard clinical procedure. Studies of proliferation, multipotent marker expression, differentiation, and gene expression were performed after culturing with dexamethasone, valproic acid, and magnesium sulfate. There were correlations between known drug effects on the human fetus and changes seen in human AFCs in culture, as well as previously undescribed observations in neural and chondrogenic inducibility. Gene expression profiles confirmed these observations. AFC culture may provide a novel method to evaluate pharmacological agents before clinical use in pregnancy.