Thymosin β4 is differentially expressed in the cerebrospinal fluid of Creutzfeldt-Jakob disease patients: a MALDI-TOF MS protein profiling study
Thymosin β4 is differentially expressed in the cerebrospinal fluid of Creutzfeldt-Jakob disease patients: a MALDI-TOF MS protein profiling study
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DOI：
10.1002/prca.200780116
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发表时间：
2009-05-01
影响因子：
2
通讯作者：
Quattrone, Aldo
中科院分区：
文献类型：
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作者：
Qualtieri, Antonio;Urso, Elena;Quattrone, Aldo
MALDI-TOF 蛋白质谱分析可以非常准确地检测复杂蛋白质混合物中的肽和小蛋白质。我们将此分析应用于 15 名克雅氏病 (CJD) 患者的脑脊液 (CSF)。我们将 11 种散发型和 4 种遗传性 CJD 形式的标准化离子信号水平与 10 名健康对照受试者和 8 名非 CJD 复发缓解型多发性硬化症患者的标准化离子信号水平进行了比较。通过这样做，我们在 CJD 样本中检测到 61 个与对照相比差异表达的离子信号。在 61 个信号中，有 3 个信号的水平显着升高，具有较高的统计显着性 (p
MALDI-TOF protein profiling analysis permits the detection of peptides and small proteins in complex protein mixtures with great accuracy. We applied this analysis to cerebrospinal fluid (C S F) from 15 patients affected by Creutzfeldt-Jakob disease (CJD). We compared the levels of the normalized ion signals of 11 sporadic and 4 genetic CJD forms with those from ten healthy control subjects and eight non-CJD relapsing-remitting multiple sclerosis patients. In so doing, we detected 61 differentially expressed ion signals in CJD samples compared to controls. Among the 61 signals, 3 signals had significantly increased levels with high statistical significance (p