Colonic Fermentation Promotes Decompression sickness in Rats.
Colonic Fermentation Promotes Decompression sickness in Rats.
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DOI：
10.1038/srep20379
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Blatteau JE
中科院分区：
文献类型：
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作者：
de Maistre S;Vallée N;Gempp E;Lambrechts K;Louge P;Duchamp C;Blatteau JE
潜水后形成大量气泡会导致减压病（DCS）。在用氢气作为氧气稀释剂的潜水过程中，通过将氢代谢微生物引入肠道来减少身体的H2负担，从而降低了DCS的风险。因此，我们着手研究导致内源性氢气产生的结肠发酵是否会促进禁食大鼠的DCS。在实验性潜水前4小时，93只禁食大鼠被强制喂食，其中一半是甘露醇，另一半是水。在强制喂食之前和之后测量呼出的氢。在高压暴露后，我们寻找DCS的迹象。强制喂食甘露醇的大鼠DCS发生率高于强制喂食水的大鼠（80%，[95%CI 56，94] vs 40%，[95%CI 19，64]，p < 0.01）。在强制喂食甘露醇的大鼠中，甲硝唑预处理降低了DCS的发生率（33%，[95%CI 15，57]，p = 0.005），同时抑制了结肠发酵（14 ± 35 ppm vs 118 ± 90 ppm，p = 0.0001）。当结肠发酵在减压阶段达到高峰时，预先摄入甘露醇增加了禁食大鼠DCS的发病率。更普遍的是，正常饮食大鼠的结肠发酵可以通过内源性氢的产生促进DCS。
Massive bubble formation after diving can lead to decompression sickness (DCS). During dives with hydrogen as a diluent for oxygen, decreasing the body’s H2 burden by inoculating hydrogen-metabolizing microbes into the gut reduces the risk of DCS. So we set out to investigate if colonic fermentation leading to endogenous hydrogen production promotes DCS in fasting rats. Four hours before an experimental dive, 93 fasting rats were force-fed, half of them with mannitol and the other half with water. Exhaled hydrogen was measured before and after force-feeding. Following the hyperbaric exposure, we looked for signs of DCS. A higher incidence of DCS was found in rats force-fed with mannitol than in those force-fed with water (80%, [95%CI 56, 94] versus 40%, [95%CI 19, 64], p < 0.01). In rats force-fed with mannitol, metronidazole pretreatment reduced the incidence of DCS (33%, [95%CI 15, 57], p = 0.005) at the same time as it inhibited colonic fermentation (14 ± 35 ppm versus 118 ± 90 ppm, p = 0.0001). Pre-diveingestion of mannitol increased the incidence of DCS in fasting rats when colonic fermentation peaked during the decompression phase. More generally, colonic fermentation in rats on a normal diet could promote DCS through endogenous hydrogen production.