Fundamental Cell Morphologies Examined With Cryo-TEM of the Species in the Novel Five Genera Robustly Correlate With New Classification in Family Mycobacteriaceae.
Fundamental Cell Morphologies Examined With Cryo-TEM of the Species in the Novel Five Genera Robustly Correlate With New Classification in Family Mycobacteriaceae.
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DOI：
10.3389/fmicb.2020.562395
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发表时间：
2020
影响因子：
5.2
通讯作者：
Mitarai S
中科院分区：
文献类型：
--
作者：
Yamada H;Chikamatsu K;Aono A;Murata K;Miyazaki N;Kayama Y;Bhatt A;Fujiwara N;Maeda S;Mitarai S
最近的一项研究基于150种分枝杆菌核心蛋白的基因组分析提出了分枝杆菌科的新分类。这些分析的结果支持分枝杆菌属内存在五个不同的单系群。也就是说，分支杆菌已被分为快速生长的类枝杆菌和枝杆菌两个新属，以及缓慢生长的枝杆菌、枝杆菌和修订的分支杆菌属三个属，其中包括所有主要的人类病原体。在此，对属于5个新属的31个种（34株）的1，816个细胞进行了冷冻TEM检查。对各属植物的细胞直径、细胞长度、细胞周长、细胞圆形度、细胞长宽比等基本形态特征进行了测量和比较。在任何两个属之间的五个参数的50个比较中，只有五个比较显示“不显著”的差异。也就是说，在缓慢生长的分枝杆菌属和分枝杆菌属之间的平均细胞直径没有显著差异（p = 0.15），在平均细胞长度方面，快速生长的类分枝杆菌属和缓慢生长的分枝杆菌属之间（p > 0.24），分支杆菌属和分枝杆菌属之间的区别分枝杆菌属与分枝杆菌属之间在平均细胞周长上差异不显著（p> 0.68），分枝杆菌属与分枝杆菌属之间在圆形度上差异不显著（p > 0.73）。其余45个比较结果表明，属间差异显著。类分枝杆菌属的平均细胞直径最长，而分枝杆菌属的平均直径最短。分枝杆菌属表现出最延长的平均细胞长度，周长和纵横比，而分枝杆菌属表现出最短的平均细胞长度，周长和纵横比。分枝杆菌属显示最高的平均细胞圆形度，而分枝杆菌属显示最低的平均细胞圆形度。这些基本的形态学资料有力地支持了分枝杆菌科的新分类，这种分类在分枝杆菌科成员的研究中是合理和有效的。由于Mycolicibacterium属和Mycobacterium属都包含了许多种，并且在每个参数上都表现出较大的显著标准差，因此这些属可以被划分为新的属，这些属在形态和致病性上表现出共同的基因型和表型。
A recent study proposed the novel classification of the family Mycobacteriaceae based on the genome analysis of core proteins in 150 Mycobacterium species. The results from these analyses supported the existence of five distinct monophyletic groups within the genus Mycobacterium. That is, Mycobacterium has been divided into two novel genera for rapid grower Mycobacteroides and Mycolicibacterium, and into three genera for slow grower Mycolicibacter, Mycolicibacillus, and an emended genus Mycobacterium, which include all the major human pathogens. Here, cryo-TEM examinations of 1,816 cells of 31 species (34 strains) belonging to the five novel genera were performed. The fundamental morphological properties of every single cell, such as cell diameter, cell length, cell perimeter, cell circularity, and aspect ratio were measured and compared between these genera. In 50 comparisons on the five parameters between any two genera, only five comparisons showed “non-significant” differences. That is, there are non-significant differences between slow grower genus Mycolicibacillus and genus Mycobacterium in average cell diameter (p = 0.15), between rapid grower genus Mycobacteroides and slow grower genus Mycobacterium in average cell length (p > 0.24), between genus Mycobacteroides and genus Mycobacterium (p > 0.68) and between genus Mycolicibacter and genus Mycolicibacillus (p > 0.11) in average cell perimeter, and between genus Mycolicibacterium and genus Mycobacterium in circularity (p > 0.73). The other 45 comparisons showed significant differences between the genera. Genus Mycobacteroides showed the longest average cell diameter, whereas the genus Mycolicibacter showed the shortest average diameter. Genus Mycolicibacterium showed the most extended average cell length, perimeter, and aspect ratio, whereas the genus Mycolicibacillus showed the shortest average cell length, perimeter, and aspect ratio. Genus Mycolicibacillus showed the highest average cell circularity, whereas genus Mycobacterium showed the lowest average cell circularity. These fundamental morphological data strongly support the new classification in the family Mycobacteriaceae, and this classification is rational and effective in the study of the members of the family Mycobacteriaceae. Because both the genus Mycolicibacterium and the genus Mycobacterium contain many species and showed larger significant standard deviations in every parameter, these genera may be divided into novel genera which show common genotype and phenotypes in morphology and pathogenicity.
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影响因子：
3.7
作者：
Joyce G;Williams KJ;Robb M;Noens E;Tizzano B;Shahrezaei V;Robertson BD
通讯作者：
Robertson BD
影响因子：
5.2
作者：
del Barrio-Duque, Alejandro;Ley, Johanna;Compant, Stephane
通讯作者：
Compant, Stephane
影响因子：
2.1
作者：
Kurita, Keisuke;Shin, Ryota;Shiomi, Daisuke
通讯作者：
Shiomi, Daisuke
影响因子：
0.8
作者：
Sanchez, Mercedes;Blesa, Alba;Berenguer, Jose
通讯作者：
Berenguer, Jose
DOI：
10.1099/ijsem.0.003920
发表时间：
2020-01-01
影响因子：
2.8
作者：
Salam, Nimaichand;Jiao, Jian-Yu;Li, Wen-Jun
通讯作者：
Li, Wen-Jun