Matrix metalloproteinase-7 expression in gastric carcinoma
Matrix metalloproteinase-7 expression in gastric carcinoma
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DOI：
10.1136/gut.39.3.444
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发表时间：
1996-09-01
期刊：
影响因子：
24.5
通讯作者：
Akiyoshi, T
中科院分区：
文献类型：
--
作者：
Honda, M;Mori, M;Akiyoshi, T
基质金属蛋白酶-7（MMP-7）与基质降解金属蛋白酶（MMPs）同属一个家族，在肿瘤细胞的侵袭和转移中起重要作用。本研究报告的MMP-7 mRNA表达水平在人胃癌和正常胃mucos. Methods从新鲜标本的47手术对原发性胃癌和相应的正常组织标本，cDNA获得逆转录（RT），然后MMP-7 mRNA检测通过聚合酶链反应。MMP-7表达的肿瘤/正常（T/N）的比例计算后校正甘油醛-3-磷酸脱氢酶作为内部control.Results-The表达校正水平的MMP-7的肿瘤大于正常粘膜中的41 47例（87%）。T/N> 2.1的13例患者胃壁浸润深度较T/N <2.0的34例患者更深，淋巴管或血管浸润更频繁。免疫组化结果显示，MMP-7主要表达于癌细胞中，在正常上皮细胞中弱表达，而在周围的间质细胞中不表达。MMP-7可能是判断胃癌生物学侵袭性的一个有用的标志物。
Background/Aims-Matrix metalloproteinase-7 (MMP-7) belongs to the same family as matrix degrading metalloproteinase (MMPs) that may play an important part in cancer cell invasion and metastasis. This study reports on the MMP-7 mRNA expression level both in human gastric carcinomas and the normal gastric mucosa.Methods-From fresh specimens of 47 surgical pairs of primary gastric carcinomas and corresponding normal tissue specimens, cDNA was obtained by reverse transcription (RT) and thereafter MMP-7 mRNAs were detected by means of a polymerase chain reaction. The tumour/normal (T/N) ratio of MMP-7 expression was calculated after correcting for glyceraldehyde-3-phosphate dehydrogenase as an internal control.Results-The expression corrected levels of MMP-7 mRNA of the tumour was greater than that of the normal mucosa in 41 of 47 cases (87%). The 13 cases whose T/N ratio was more than 2.1 showed a deeper invasion of the gastric wall, and more frequent lymphatic or vascular permeations than the 34 cases whose T/N ratio was less than 2.0. An immunohistochemical study showed that MMP-7 was predominantly expressed in the cancer cells, weakly expressed in normal epithelial cells, and not expressed in the surrounding stromal cells.Conclusions-These findings suggest that the overexpression of MMP-7 may thus play an important part in tumour invasion in gastric carcinomas while, in addition, MMP-7 may also prove to be a useful marker for determining the biological aggressiveness of gastric carcinoma.