INVITRO SUSCEPTIBILITY OF VARICELLA-ZOSTER VIRUS TO ACYCLOVIR
INVITRO SUSCEPTIBILITY OF VARICELLA-ZOSTER VIRUS TO ACYCLOVIR
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DOI：
10.1128/aac.18.3.443
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发表时间：
1980-01-01
影响因子：
4.9
通讯作者：
ELION, GB
中科院分区：
文献类型：
--
作者：
BIRON, KK;ELION, GB
用空斑减少法测定了5株水痘-带状疱疹病毒对阿昔洛韦的体外敏感性。阿昔洛韦的50%有效剂量在2.06-6.28微米之间，平均3.65微米。当药物剂量超过斑块减少的50%有效剂量时，可实现不可逆的抑制斑块形成，但对细胞无毒。关于水痘带状疱疹病毒和1型和2型单纯疱疹病毒对阿昔洛韦的相对体外敏感性的研究表明，水痘带状疱疹病毒对阿昔洛韦的敏感性降低了2-8倍。比较了阿昔洛韦和其他几种核苷类似物对水痘带状疱疹病毒的体外抗病毒效力。对水痘带状疱疹病毒感染的WI-38细胞中阿昔洛韦代谢的分析表明，与1型和2型单纯疱疹病毒一样，药物三磷酸形式的形成是病毒感染特有的。
The in vitro susceptibility of 5 strains of varicella-zoster virus to acyclovir was examined by the plaque-reduction method in human diploid lung cells. The 50% effective doses of acyclovir ranged from 2.06-6.28 .mu.M in a 7 day assay, with a mean of 3.65 .mu.M. Irreversible inhibition of plaque formation was achieved by drug doses exceeding the 50% effective dose for plaque reduction but nontoxic to the cells. Studies on the relative in vitro susceptibility of varicella-zoster virus and herpes simplex virus types 1 and 2 to acyclovir suggested that varicella-zoster virus is 2- to 8-fold less susceptible to the drug. The antiviral potency of acyclovir for varicella-zoster virus in vitro was compared with that of several other nucleoside analogs. Analysis of the metabolism of acyclovir in varicella-zoster virus-infected WI-38 cells revealed that, as with herpes simplex virus types 1 and 2, the formation of the triphosphate forms of the drug is specific to viral infection.