Variovorax soli sp nov., isolated from greenhouse soil
Variovorax soli sp nov., isolated from greenhouse soil
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DOI：
10.1099/ijs.0.64390-0
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Stackebrandt, Erko
中科院分区：
文献类型：
--
作者：
Kim, Byung-Yong;Weon, Hang-Yeon;Stackebrandt, Erko
对从温室土壤中分离到的一株革兰氏阴性、杆状、非芽胞形成细菌GH9-3(T)进行了多相研究。这种新型微生物在10-35℃、0-3%的氯化钠和pH为5-9的环境中生长。其主要成分为泛醌8(Q-8)，C-16：0，特征3，C-17：0环和C-18：1欧米伽7c为主要脂肪酸(占总脂肪酸的87.4%)。DNA G+C含量为67.1%。对菌株GH9-3(T)的16SRRNA基因序列分析表明，该菌株属于Variovorax簇，与Variovorax paroxus AM 12373(T)(98(.)3%)和Variovorax dokdonensis DS-43(T)(98(.)0%)具有最高的序列相似性。菌株GH9-3T与反常弧菌DSM 30034(T)和DOKDONASS-43(T)的杂交值分别为38%和29%。根据表型特征、化学分类和系统发育特征，提出菌株GH9-3(T)为变种属一新种，命名为Variovorax soli sp.11月模式菌株为GH9-3(T)(=KAcc 11579(T)=Dsm 18216(T))。
A Gram-negative, rod-shaped, non-spore-forming bacterium, strain GH9-3(T), isolated from greenhouse soil, was investigated in a polyphasic study. The novel organism grew at 10-35 degrees C, 0-3 % NaCl and pH 5-9. It had ubiquinone 8 (Q-8) as the predominant isoprenoid quinone and possessed C-16:0, summed feature 3, C-17:0 cyclo and C-18:1 omega 7c as the major fatty acids (together representing 87(.)4% of the total). The DNA G + C content was 67.1 mol%. 16S rRNA gene sequence analysis of strain GH9-3(T) showed that it grouped within the Variovorax cluster, with highest sequence similarities to Variovorax paradoxus AM 12373(T) (98(.)3%) and Variovorax dokdonensis DS-43(T) (98(.)0%). DNA-DNA hybridization values between strain GH9-3 T and V. paradoxus DSM 30034(T) and V dokdonensis DS-43(T) were 38 and 29 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic features, it is proposed that strain GH9-3(T) represents a novel species of the genus Variovorax with the name Variovorax soli sp. nov. The type strain is GH9-3(T) (= KACC 11579(T) =DSM 18216(T)).