Vitamin K epoxide reductase: evidence that vitamin K dihydroquinone is a product of vitamin K epoxide reduction.
Vitamin K epoxide reductase: evidence that vitamin K dihydroquinone is a product of vitamin K epoxide reduction.
复制标题
维生素 K 环氧化物还原酶：证据表明维生素 K 二氢醌是维生素 K 环氧化物还原的产物。
DOI：
10.1016/0006-291x(81)90908-6
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发表时间：
1981
影响因子：
3.1
通讯作者：
Eugene G. Sander
中科院分区：
文献类型：
--
作者：
Paula A. Sherman;Eugene G. Sander
维生素K环氧化物还原酶是一种以二硫苏糖醇作为还原当量的主要或次要来源来催化维生素K环氧化物还原的双组分酶活性。高效液相色谱分析系统表明，维生素K的二氢对苯二酚形式除苯二酚外，还是一种反应产物。羧甲基纤维素层析表明，相同的胞内蛋白质组分可能参与了二硫苏糖醇支持的维生素K环氧化物还原、二硫苏糖醇支持的维生素K醌还原和NADH支持的二氯苯酚-吲哚酚还原。
Vitamin K epoxide reductase is a two component enzyme activity which catalyzes the reduction of Vitamin K epoxide using dithiothreitol as either a primary or secondary source of reducing equivalents. A high performance liquid chromatographic assay system indicates that in addition to the quinone, the dihydroquinone form of Vitamin K is a reaction product. Carboxymethyl cellulose chromatography suggests that the same cytosolic protein fraction may be involved in the dithiothreitol-supported reduction of Vitamin K epoxide, the dithiothreitol-supported reduction of Vitamin K quinone and the NADH-supported reduction of dichlorophenol-indophenol.