BILIRUBIN INHIBITS THE ACTIVATION OF SUPEROXIDE-PRODUCING NADPH OXIDASE IN A NEUTROPHIL CELL-FREE SYSTEM
BILIRUBIN INHIBITS THE ACTIVATION OF SUPEROXIDE-PRODUCING NADPH OXIDASE IN A NEUTROPHIL CELL-FREE SYSTEM
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DOI：
10.1016/0167-4838(91)90478-i
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发表时间：
1991-02-15
期刊：
影响因子：
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通讯作者：
MINAKAMI, S
中科院分区：
文献类型：
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作者：
KWAK, JY;TAKESHIGE, K;MINAKAMI, S
我们研究了胆红素对十二烷基硫酸钠诱导的猪中性粒细胞的膜和胞浆组分组成的无细胞系统中的NADPH依赖的超氧化物产生的影响。 预孵育的细胞溶质馏分与胆红素之前，加入十二烷基硫酸钠导致的时间和剂量依赖性的抑制超氧化物的产生，而预孵育的膜馏分与四吡咯没有导致抑制。 当在反应开始后加入颜料时，正在进行的生产不受添加的影响。 其他四吡咯类化合物，如氯化血红素、原卟啉和胆绿素，也能抑制其生成。 结果表明，胆红素抑制超氧化物产生NADPH氧化酶的激活过程中，通过降低细胞质组分的效力，其抑制作用似乎是由于四吡咯的疏水性。
We studied the effect of bilirubin on the NADPH-dependent superoxide production induced by sodium dodecyl sulfate in a cell-free system consisting of the membrane and cytosolic fractions of pig neutrophils. Preincubation of the cytosolic fraction with bilirubin before the addition of sodium dodecyl sulfate resulted in the time- and dose-dependent inhibition of the superoxide production while the preincubation of the membrane fraction with the tetrapyrrole did not result in the inhibition. When the pigment was added after the initiation of the reaction, the ongoing production was not affected by the addition. Other tetrapyrroles, such as hemin, protoporphyrin and biliverdin, also inhibited the production. The results indicate that bilirubin inhibits the activation process of the superoxide producing NADPH oxidase by decreasing the potency of the cytosolic fraction and its inhibitory effect seems to be due to the hydrophobic nature of the tetrapyrrole.