Effects of cold- and heat hardening on thermal resistance in Drosophila melanogaster.
Effects of cold- and heat hardening on thermal resistance in Drosophila melanogaster.
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DOI：
10.1016/s0022-1910(03)00095-7
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发表时间：
2003-08
影响因子：
2.2
通讯作者：
Margit Sejerkilde;J. G. Sørensen;V. Loeschcke
中科院分区：
文献类型：
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作者：
Margit Sejerkilde;J. G. Sørensen;V. Loeschcke
研究了冷硬化和热硬化对果蝇低温和高温胁迫抗性的影响。在所有选择条件下，当硬化类型与应力相同时，硬化效果均为正。在选择抗寒品系和相应对照中，进一步研究了冷硬化对热胁迫后存活的影响。交叉保护效应（冷硬化后耐热性增加）是存在的，这种效应在抗冷性选择的品系中比在对照组中要低。检测了非致死冷硬化诱导的Hsp70表达水平，结果表明冷硬化诱导了Hsp70的表达。结果表明，交叉保护效应至少部分是由于冷暴露诱导的Hsp70表达。
The effects of cold- and heat hardening on resistance to both low and high temperature stress was examined in Drosophila melanogaster lines selected for resistance to either cold or heat. The hardening effect was positive when the hardening was of the same type as the stress in all selection regimes. The effect of cold hardening on survival after heat stress was further examined in the lines selected for cold resistance and corresponding controls. A cross-protection effect (increased heat resistance after cold hardening) was present and this effect was lower in the lines selected for resistance to cold than in the controls. The level of Hsp70 expression induced by a non-lethal cold hardening was examined, showing that cold hardening induced Hsp70 expression. The results suggest that the cross-protection effect is at least partly due to Hsp70 expression induced by cold exposure.