A peptide-DNA hybrid bio-nanomicelle and its application for detection of caspase-3 activity.
A peptide-DNA hybrid bio-nanomicelle and its application for detection of caspase-3 activity.
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肽-DNA杂化生物纳米胶束及其在caspase-3活性检测中的应用
DOI：
10.3389/fchem.2022.1005315
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发表时间：
2022
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
基于核酸、多肽、聚糖、脂类等生物材料的生物纳米胶束在生物分析领域发展迅速。虽然DNA和肽具有独特的优势，但遗憾的是，很少有将DNA与肽整合的生物纳米胶束。本文设计了一种肽-DNA杂化生物纳米胶束，用于caspase-3的活性检测。其检测机制是基于caspase-3对肽底物的特异性识别和切割，具有较高的灵敏度和选择性。在最佳条件下，利用我们设计的生物纳米胶束可以实现caspase-3活性的检测，检测限为0.72 nM。此外，该方法也成功地应用于检测caspase-3的细胞裂解样品后，诱导凋亡。
Bio-nanomicelles based on biomaterials such as nucleic acids, peptides, glycans, and lipids have developed rapidly in the field of bioanalysis. Although DNA and peptides have unique advantages, unfortunately, there are few bio-nanomicelles integrating DNA with peptides. Here, we designed a peptide-DNA hybrid bio-nanomicelle for the activity detection of caspase-3. The detection mechanism is based on caspase-3 specific recognition and cleavage of peptide substrates, which owns high sensitivity and selectivity. Under optimal conditions, the detection of caspase-3 activity can be achieved using our designed bio-nanomicelles and the detection limit is 0.72 nM. Furthermore, the proposed method was also successfully applied for the detection of caspase-3 in cell lysate samples after apoptosis-inducing.