Identification of novel rainbow trout (Onchorynchus mykiss) chemokines, CXCd1 and CXCd2: mRNA expression after Yersinia ruckeri vaccination and challenge
Identification of novel rainbow trout (Onchorynchus mykiss) chemokines, CXCd1 and CXCd2: mRNA expression after Yersinia ruckeri vaccination and challenge
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DOI：
10.1007/s00251-006-0084-7
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发表时间：
2006-03
期刊：
影响因子：
3.2
通讯作者：
G. Wiens;G. Glenney;S. Lapatra;T. Welch
中科院分区：
文献类型：
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作者：
G. Wiens;G. Glenney;S. Lapatra;T. Welch
趋化因子在正常和炎症条件下控制白细胞运输中发挥重要作用。在人类和小鼠基因组中已鉴定出 16 种 CXC 趋化因子，而据报道的硬骨鱼 CXC 趋化因子要少得多。在这里，我们描述了鳟鱼 (Onchorynchus mykiss) CXC 趋化因子的一个新分支，命名为 Onmy CXCd，并且我们鉴定了一个新基因 CXCd1 和一个假定的重复基因 CXCd2。鳟鱼CXCd蛋白含有112个氨基酸，CXCd1基因由四个外显子和三个内含子组成。在皮肤、鳃、内脏脂肪和后肾组织中检测到组成型CXCdmRNA表达，而在前肾和脾脏中存在低转录水平。接种疫苗后 1 天，脾CXCd 转录本丰度增加（四倍），并在接种后 7 天降至基础水平。用活的鲁氏耶尔森氏菌进行攻击可诱导脾脏中鳟鱼CXCdRNA 的表达高达九倍。存活数Y。 ruckeri与CXCd基因转录丰度显着相关（P=0.0051，Spearman相关性0.497，n=30条鱼），并且细菌负荷最高的鱼具有最高的CXCd表达。相反，感染少量 Y 的鱼中促炎细胞因子 IL-1-β2 mRNA 水平升高。 ruckeri，而在严重感染的鱼中减少。CXCdmRNA 表达在感染传染性造血坏死病毒的虹鳟鱼中没有增加，表明上调可能是病原体特异性的。总而言之，这些结果表明CXCd转录物升高遵循对Y的促炎细胞因子反应。 ruckeriand 可能是暴露的相关免疫标记物。
Chemokines play important roles in controlling leukocyte trafficking under normal and inflammatory conditions. Sixteen CXC chemokines have been identified in the human and mouse genomes, while considerably fewer teleost fish CXC chemokines have been reported. Here, we describe a novel clade of trout (Onchorynchus mykiss) CXC chemokines, designatedOnmy CXCd, and we identify a novel gene,CXCd1, and a putative duplicate,CXCd2. The trout CXCd proteins contain 112 amino acids and theCXCd1gene is comprised of four exons and three introns. ConstitutiveCXCdmRNA expression was detected in skin, gill, visceral fat, and posterior kidney tissues, while low transcript levels were present in the anterior kidney and spleen. SpleenCXCdtranscript abundance increased 1 day after bath vaccination (fourfold) and subsided to basal levels by 7 days postvaccination. Challenge with viableYersinia ruckeriinduced expression of troutCXCdRNA up to ninefold in the spleen. The number of viableY. ruckeriwere significantly correlated withCXCdgene transcript abundance (P=0.0051, Spearman correlation 0.497,n=30 fish), and fish with the highest bacterial loads had the highestCXCdexpression. In contrast, pro-inflammatory cytokine IL-1-β2 mRNA levels were elevated in fish infected with low numbers ofY. ruckeri, while diminishing in heavily infected fish.CXCdmRNA expression was not increased in rainbow trout infected with infectious hematopoietic necrosis virus, suggesting that up-regulation may be pathogen-specific. Taken together, these results indicate thatCXCdtranscript elevation follows the pro-inflammatory cytokine response toY. ruckeriand may be a relevant immunological marker of exposure.