REGULATION OF HUMAN MELANOCYTE GROWTH, DENDRICITY, AND MELANIZATION BY KERATINOCYTE DERIVED FACTORS
REGULATION OF HUMAN MELANOCYTE GROWTH, DENDRICITY, AND MELANIZATION BY KERATINOCYTE DERIVED FACTORS
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DOI：
10.1111/1523-1747.ep12709595
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发表时间：
1989-04-01
影响因子：
6.5
通讯作者：
GILCHREST, BA
中科院分区：
文献类型：
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作者：
GORDON, PR;MANSUR, CP;GILCHREST, BA
表皮色素沉着涉及黑素细胞中黑素的合成及其转移到周围的角质形成细胞，在那里它起光保护作用。为了研究角质形成细胞在调节色素沉着中的可能作用，将人角质形成细胞在确定的培养基中孵育24小时，然后将其转移到纯的人黑素细胞培养物中。1周后，条件培养基使黑素细胞产量增加4倍，总黑素增加7倍.增加的黑素细胞密度在24小时内也清晰可见。角质形成细胞条件培养基的超滤表明大约一半的生长促进活性以及大部分的树突和黑化刺激活性是低分子量的（
Epidermal pigmentation involves the synthesis of melanin in melanocytes and its transfer to surrounding keratinocytes, where it functions in photoprotection. To investigate the possible role of the keratinocyte in regulating pigmentation, human heratinocytes were incubated for 24 h in a defined culture medium, which was then transferred to pure human melanocyte cultures. After 1 week, the conditioned medium produced a fourfold increase in melanocyte yield and a seven- fold increase in total melanin. Increased melanocyte dendricity was clearly visible within 24 h as well. Ultrafiltration of the keratinocyte-conditioned medium suggested approximately one-half of the growth promoting activity as well as most of the dendricity and melanization stimulating activities were of low molecular weight (