Ascending collaterals of medullary barosensitive neurons and C1 cells in rats.
Ascending collaterals of medullary barosensitive neurons and C1 cells in rats.
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大鼠髓质压力敏感神经元和 C1 细胞的升络。
DOI：
10.1152/ajpregu.1990.258.4.r1051
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Guyenet,PG
中科院分区：
文献类型：
--
作者：
Haselton,JR;Guyenet,PG
在大鼠身上进行了三标记实验，以确定延髓吻侧腹外侧（RVLM）网状脊髓C1肾上腺素能神经元是否也支配脑桥背外侧（LPBN/KF）、中脑中央灰色区（CG）和下丘脑（LH）。10%投射到LPBN/KF的RVLM神经元对酪氨酸羟化酶（TH）有免疫反应，50%投射到肾盂旁灰（PAG）或LH的RVLM神经元对TH有免疫反应。大量由LPBN/KF或PAG逆行标记的RVLM th免疫反应神经元（分别为65.5%和28.5%）也从脊髓逆行标记。由LH标记的RVLM th免疫反应神经元中有一小部分也来自脊髓（13.1%）。绝大多数共化RVLM神经元具有TH免疫反应性（LPBN/KF为50%，PAG为82%，LH为84%），其余可能为非肾上腺素能神经元。此外，研究人员还利用反向（AD）定位技术确定RVLM网状脊髓压力敏感神经元（假定为血管舒张性交感兴奋细胞，共106个样本）是否在解剖上向髓上区发送轴突侧支。20%的样本细胞（n = 21）也从20个髓上部位中的1个被AD激活。综合方法支持以下结论：1)RVLM压力敏感神经元包括表型上的肾上腺素能细胞，2)RVLM压力敏感细胞并不代表一个专门为交感神经节前神经元提供兴奋驱动的输出通路。
Triple-label experiments were conducted in rats to determine whether reticulospinal C1 adrenergic neurons of rostral ventrolateral medulla (RVLM) also innervate dorsolateral pons (LPBN/KF), mesencephalic central gray area (CG), and hypothalamus (LH). Ten percent of RVLM neurons that project to LPBN/KF were immunoreactive for tyrosine hydroxylase (TH), and 50% of those projecting to either pariaqueductal gray (PAG) or LH were TH immunoreactive. A large proportion of RVLM TH-immunoreactive neurons that were retrogradely labeled from either LPBN/KF or PAG (65.5 and 28.5%, respectively) were retrogradely labeled from the spinal cord as well. A smaller fraction of RVLM TH-immunoreactive neurons that were labeled from LH were also labeled from the spinal cord (13.1%). The vast majority of the cohaterized RVLM neurons were TH immunoreactive (LPBN/KF 50%; PAG 82%; LH 84%), and the remaining ones may be nonadrenergic neurons. Antidromic (AD) mapping techniques were also used to determine if RVLM reticulospinal barosensitive neurons (putative vasomotor sympathoexcitatory cells, total sample of 106) send axonal collaterals to the supramedullary areas investigated anatomically. Twenty percent of the cells sampled (n = 21) were also AD activated from 1 of 20 supramedullary sites. The combined approaches support the following conclusions: 1) RVLM barosensitive neurons include phenotypically adrenergic cells, and 2) RVLM barosensitive cells do not represent an output pathway solely dedicated to providing an excitatory drive to sympathetic preganglionic neurons.