Caffeine attenuates liver fibrosis via defective adhesion of hepatic stellate cells in cirrhotic model
Caffeine attenuates liver fibrosis via defective adhesion of hepatic stellate cells in cirrhotic model
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DOI：
10.1111/jgh.12317
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发表时间：
2013-12-01
影响因子：
4.1
通讯作者：
Yoon, Byung Chul
中科院分区：
文献类型：
--
作者：
Shim, Sung Gon;Jun, Dae Won;Yoon, Byung Chul
Background and AimSeveral epidemiological studies have shown that coffee intake attenuates the progression of liver fibrosis; however, the mechanism is unclear.AimsWe investigated the direct effects of caffeine on hepatic stellate cells (HSCs) and assessed whether caffeine attenuated intrahepatic fibrosis in rat model of liver cirrhosis.MethodsHuman hepatic stellate cell line, an immortalized human HSCs line, was used in in vitro assay system. Cell migration and proliferation were assessed in presence of various caffeine concentrations (0, 1, 5, and 10mmol), and levels of procollagen type Ic and -smooth muscle actin (-SMA) were measured by Western blot. Severity of liver inflammation and fibrosis were compared between thioacetamide-treated rats with and without caffeine supplementation.ResultsCaffeine increased HSCs apoptosis and intracellular F-actin and cyclic adenosine monophosphate expression. Caffeine also inhibited procollagen type Ic and -SMA expression in a dose- and time-dependent manner. In rat model, caffeine decreased periportal inflammation, levels of inflammatory cells (1.40.52 vs 2.6 +/- 0.46, P
