Location of variable and conserved epitopes among the multiple serotypes of streptococcal M protein.
Location of variable and conserved epitopes among the multiple serotypes of streptococcal M protein.
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DOI：
10.1084/jem.161.3.623
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发表时间：
1985-03-01
期刊：
影响因子：
--
通讯作者：
Fischetti VA
中科院分区：
文献类型：
--
作者：
Jones KF;Manjula BN;Johnston KH;Hollingshead SK;Scott JR;Fischetti VA
在主要局限于纤维状A组链球菌M蛋白的氨基末端区域的研究中，在M血清型中仅观察到有限的免疫交叉反应。在这项调查中，两个单克隆抗体产生的几乎整个M6分子（LysM6）被用来确定M血清分型菌株之间的交叉反应的程度和本地化的M分子上的表位。集落印迹和免疫印迹分析显示，负责测试的56种不同M血清型菌株中的5种菌株之间的交叉反应的表位位于分子的氨基末端一半，远离细胞表面。相比之下，一个更常见的交叉反应表位，与56株中的20株反应，位于M分子的中间附近。这些研究还表明，更保守的决定簇，位于更接近细胞表面，是可接近的免疫系统，甚至在整个生物体，因此，可能是有用的设计手段，以防止感染的多组A链球菌M血清型。
In studies primarily confined to the amino-terminal region of the fibrillar group A streptococcal M protein, only limited immunological crossreactions have been observed among M serotypes. In this investigation, two monoclonal antibodies generated against nearly the entire M6 molecule (LysM6) were used to determine the extent of crossreactions among M serotyping strains and to localize their epitopes on the M molecule. Colony blot and immunoblot analyses revealed that an epitope responsible for crossreactions among 5 of the 56 strains of different M serotypes tested is located in the amino- terminal half of the molecule, distal to the cell surface. In contrast, a more common crossreactive epitope, reacting with 20 of the 56 strains, is located near the middle of the M molecule. These studies also reveal that the more conserved determinant, located more proximally to the cell surface, is accessible to the immune system, even on the whole organism, and, thus, may be useful in devising means to protect against infections by multiple group A streptococcal M serotypes.