PULSATILE SOMATOSTATIN RELEASE FROM THE MEDIAN-EMINENCE OF THE UNANESTHETIZED RAT AND ITS RELATIONSHIP TO PLASMA GROWTH-HORMONE LEVELS
PULSATILE SOMATOSTATIN RELEASE FROM THE MEDIAN-EMINENCE OF THE UNANESTHETIZED RAT AND ITS RELATIONSHIP TO PLASMA GROWTH-HORMONE LEVELS
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DOI：
10.1210/endo-109-5-1739
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发表时间：
1981-01-01
期刊：
影响因子：
4.8
通讯作者：
ARNOLD, MA
中科院分区：
文献类型：
--
作者：
KASTING, NW;MARTIN, JB;ARNOLD, MA
推拉灌注技术与敏感的生长抑素[放射免疫测定法]相结合，以确定在体内免疫反应生长抑素（IRS）释放的正中隆起（ME）的自由行为的大鼠。IRS释放与血浆GH [生长激素]水平进行比较。ME灌注液中IRS的浓度表明脉冲释放，峰间间隔为λ。1小时在具有正常血浆GH脉冲和正常血浆PRL水平的大鼠中，IRS分泌范围为10-100 pg/15 min。在具有抑制GH分泌和高血浆PRL水平的大鼠中，IRS分泌更大，范围为50-500 pg/15 min。ME灌注液中IRS水平的升高通常与血浆中GH水平的升高一致，提示生长抑素可能通过短环负反馈机制调节GH的释放。IRS的凝胶色谱从灌注液表明，生长抑素被释放在一个以上的MW形式。
The technique of push-pull perfusion was combined with a sensitive somatostatin [radioimmunoassay] to determine in vivo immunoreactive somatostatin (IRS) release from the median eminence (ME) of the freely behaving rat. IRS release was compared to plasma GH [growth hormone] levels. Concentrations of IRS in ME perfusates indicated pulsatile release, with an interpeak interval of .apprx. 1 h. In rats with normal plasma GH pulses and normal plasma PRL levels, IRS secretion ranged from 10-100 pg/15 min. In rats with suppressed GH secretion and high plasma PRL levels, IRS secretion was greater, ranging between 50-500 pg/15 min. A rise in the level of IRS in the ME perfusate often coincided with an increase in GH levels in plasma, suggesting that somatostatin may act by a short-loop negative feedback mechanism to regulate GH release. Gel chromatography of IRS from the perfusate suggested that somatostatin is released in more than one MW form.