Mechanism of capillary electrophoresis enantioseparations using a combination of an achiral crown ether plus cyclodextrins
Mechanism of capillary electrophoresis enantioseparations using a combination of an achiral crown ether plus cyclodextrins
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DOI：
10.1016/s0021-9673(97)00826-1
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发表时间：
1998-01-09
影响因子：
4.1
通讯作者：
Chang, SSC
中科院分区：
文献类型：
--
作者：
Armstrong, DW;Chang, LW;Chang, SSC
在基于环糊精的拆分中加入非手性冠醚(18-冠-6)可以显著影响含有伯胺官能团的有机外消旋的毛细管电泳法(CE)对映体拆分。在大多数情况下，观察到对映体分离的增强。然而，也有一些情况下，添加18-冠-6不利于基于环糊精的CE对映体分离。考察了两种络合添加剂的浓度、pH值、钾离子加入量对络合效果的影响。当18-冠-6加入到RUN缓冲液中时，可以用同时形成双包涵体络合物的特殊三体络合物来解释观察到的增强和降低的对映体选择性。(C)1998年爱思唯尔科学公司。
The addition of an achiral crown ether (18-crown-6) to a cyclodextrin-based separation can significantly affect the capillary electrophoresis (CE) enantioresolution of organic racemates that contain a primary amine functional group. In most cases an enhancement of the enantioseparation was observed. However, there are also cases where the addition of 18-crown-6 was detrimental to a cyclodextrin-based CE enantioseparation. The effect of concentration of the two complexing additives as well as the effect of pH and added potassium ion were examined. A specific three-body complex involving simultaneous, dual inclusion complex formation can be used to explain both the enhanced and diminished enantioselectivities observed when 18-crown-6 is added to the run buffer. (C) 1998 Elsevier Science B.V.