Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
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DOI：
10.1016/s0092-8674(00)00084-2
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发表时间：
2000-09-01
期刊：
影响因子：
64.5
通讯作者：
Yonath, A
中科院分区：
文献类型：
--
作者：
Schluenzen, F;Tocilj, A;Yonath, A
核糖体小亚基在翻译过程中执行遗传信息的解码。嗜热栖热菌的结构表明，定位mRNA和三种tRNA的解码中心完全由RNA构成。mRNA通道的入口将在闩锁状接触关闭时包围信息，并有助于持续性和保真度。延伸的RNA螺旋元件纵向贯穿身体，传递结构变化，将颗粒远端的事件与解码区的mRNA易位周期相关联。追踪了96%的核苷酸，并确定了所有蛋白质的主要折叠。后者要么是外围的，要么似乎是作为连接器。有些可能有助于易位的方向性。
The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.