19-nor-DOC biosynthesis in the isolated perfused rat kidney.
19-nor-DOC biosynthesis in the isolated perfused rat kidney.
复制标题
离体灌注大鼠肾脏中的 19-nor-DOC 生物合成。
DOI：
10.1016/0960-0760(91)90352-6
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Lieberthal,W
中科院分区：
文献类型：
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作者：
Azar,ST;Melby,JC;Holbrook,MM;Wilson,TE;LaRaia,J;Lieberthal,W
19-去甲-脱氧皮质酮（19-nor-DOC）是一种有效的盐保留和高血压盐皮质激素，通过尿液排出。虽然19-nor-DOC的前体19-oxo-DOC是由肾上腺皮质产生的，但在肾上腺中不会转化为19-nor-DOC。我们已经检验了19-nor-DOC是由肾脏中的前体合成的假设。将19-oxo-DOC以10 μM的浓度加入到离体大鼠肾脏制备物（n = 5）的灌注液中。在加入19-oxo-DOC后灌注1小时期间，71 ± 6%的前体转化为19-oic-DOC，19-nor-DOC的直接前体，8.3 ± 1.8%转化为19-nor-DOC。这是第一个明确的证据表明，19-nor-DOC是从肾上腺前体在肾脏中产生。
19-nor-deoxycorticosterone (19-nor-DOC) is a potent salt retaining and hypertensinogenic mineralocorticoid that is excreted in the urine. While the precursor of 19-nor-DOC, 19-oxo-DOC, is produced by the adrenal cortex, conversion to 19-nor-DOC does not occur in the adrenal gland. We have examined the hypothesis that 19-nor-DOC is synthesized from precursors in the kidney. 19-oxo-DOC was added to the perfusate of isolated rat kidney preparations (n = 5) at a concentration of 10 μM. During 1 h of perfusion following addition of 19-oxo-DOC, 71 ± 6% of the precursor was converted to 19-oic-DOC, an immediate precursor of 19-nor-DOC, and 8.3 ± 1.8% was converted to 19-nor-DOC. This represents the first definitive evidence that 19-nor-DOC is produced in the kidney from adrenal precursors.