Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution
Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution
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DOI：
10.1534/genetics.117.300344
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发表时间：
2018-02-01
期刊：
影响因子：
3.3
通讯作者：
Jiang, Jiming
中科院分区：
文献类型：
--
作者：
Braz, Guilherme T.;He, Li;Jiang, Jiming
真核生物核型的发展依赖于物种中单个染色体的鉴定，这只在有限的时间内完成。真核生物染色体组型的建立依赖于单个染色体的鉴定，这对于大多数非模式植物和动物物种来说是一个重大挑战。我们开发了一种新的染色体识别系统，通过选择和标记位于每个染色体上特定区域的寡核苷酸（oligos）。我们基于马铃薯基因组中的单拷贝DNA序列选择了一组54，672个寡核苷酸（45 nt）。这些寡核苷酸产生了26个不同的FISH信号，这些信号可以用作条形码或带型，以独特地标记来自二倍体和多倍体（4x和6x）马铃薯物种的12条染色体中的每一条。值得注意的是，相同的条形码可以用来识别12个同源染色体之间的远距离相关的茄属物种，包括番茄和茄子。基于单独鉴定的染色体，在已经分化>15 MY的6个茄属物种中建立了准确的核型。这六个物种保持了相似的核型，然而，对FISH信号条形码的修改导致在Solanum etuberosum和S. caripense。我们还通过基于寡核苷酸的染色体绘制验证了这些易位。我们表明，基于寡核苷酸的FISH技术是强大的新工具，染色体鉴定和核型分析研究，特别是对非模式植物物种。
Development of a eukaryotic karyotype relies on identification of individual chromosomes in the species, which has been accomplished only in a limited... Developing the karyotype of a eukaryotic species relies on identification of individual chromosomes, which has been a major challenge for most nonmodel plant and animal species. We developed a novel chromosome identification system by selecting and labeling oligonucleotides (oligos) located in specific regions on every chromosome. We selected a set of 54,672 oligos (45 nt) based on single copy DNA sequences in the potato genome. These oligos generated 26 distinct FISH signals that can be used as a bar code or banding pattern to uniquely label each of the 12 chromosomes from both diploid and polyploid (4x and 6x) potato species. Remarkably, the same bar code can be used to identify the 12 homeologous chromosomes among distantly related Solanum species, including tomato and eggplant. Accurate karyotypes based on individually identified chromosomes were established in six Solanum species that have diverged for >15 MY. These six species have maintained a similar karyotype; however, modifications to the FISH signal bar code led to the discovery of two reciprocal chromosomal translocations in Solanum etuberosum and S. caripense. We also validated these translocations by oligo-based chromosome painting. We demonstrate that the oligo-based FISH techniques are powerful new tools for chromosome identification and karyotyping research, especially for nonmodel plant species.