A new method for assessing the % of spirochaetes in subgingival plaque.
A new method for assessing the % of spirochaetes in subgingival plaque.
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%20新%20方法%20用于%20评估%20%20龈下%20牙菌斑中%20螺旋菌%20的%20%%20。
DOI：
10.1111/j.1600-051x.1986.tb00860.x
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发表时间：
1986
影响因子：
6.7
通讯作者：
T. Watkins
中科院分区：
文献类型：
--
作者：
T. Macfarlane;J. McCourtie;T. Watkins
本文介绍一种新的龈下菌斑螺旋体计数方法。该技术涉及负染色制备，暗视野显微镜和图像分析系统的组合使用。该方法的优点是：涂片在取样后2天内不需要制作，可在制备后1年内进行显微镜检查;计数的重现性大于90%，数据可计算机化。新的方法应被证明是有用的，在常规评估龈下菌斑的患者与各种形式的牙周炎，并在流行病学调查。
A new method for counting the numbers of spirochaetes in subgingival plaque is described. The technique involves the combined use of a negative stained preparation, dark field microscopy, and an image analysing system. Advantages of the method include: smears need not be made until 2 days after sampling, and can be examined microscopically for up to 1 year after preparation; reproducibility of counts are more than 90% and the data can be computerised. The new method should prove useful in the routine assessment of subgingival plaque from patients with various forms of periodontitis, and in epidemiological surveys.