PATTERNS OF ELEVATED FREE CALCIUM AND CALMODULIN ACTIVATION IN LIVING CELLS
PATTERNS OF ELEVATED FREE CALCIUM AND CALMODULIN ACTIVATION IN LIVING CELLS
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DOI：
10.1038/359736a0
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发表时间：
1992-10-22
期刊：
影响因子：
64.8
通讯作者：
TAYLOR, DL
中科院分区：
文献类型：
--
作者：
HAHN, K;DEBIASIO, R;TAYLOR, DL
细胞内信号和蛋白质效应物的时间和空间动态被定义为在活细胞内使用荧光试剂进行成像的结果1-5。我们描述了一种新型荧光类似物2，称为光学生物传感器6，它通过对蛋白质传感器7的影响来感知化学或分子事件。这类新型指示剂的一个例子是 MeroCaM，它是一种环境敏感的荧光团，当它附着在钙调蛋白上时，可以反映钙调蛋白在体外被钙激活2。我们在此报告，在静态成纤维细胞的血清刺激期间，游离钙和 MeroCaM 激活的增加发生在同一时期。 MeroCaM 激活还与伤口愈合过程中游离钙增加和横向纤维收缩的空间模式相关1,8-10。最后，在伤口愈合的后期，迁移的成纤维细胞表现出从前到后游离钙和 MeroCaM 激活的梯度增加。
THE temporal and spatial dynamics of intracellular signals and protein effectors are being defined as a result of imaging using fluorescent reagents within living cells1-5. We have described a new class of fluorescent analogues2 termed optical biosensors6, which sense chemical or molecular events through their effects on protein transducers7. One example of this new class of indicators is MeroCaM, an environmentally sensitive fluorophore which when it is attached to calmodulin reflects the activation of calmodulin by calcium in vitro2. We report here that the rise in free calcium and MeroCaM activation occur in the same period during serum stimulation of quiescent fibroblasts. MeroCaM activation also correlates with the spatial pattern of increased free calcium and the contraction of transverse fibres during wound healing1,8-10. Finally, migrating fibroblasts in the later stages of wound-healing exhibit an increasing gradient of free calcium and MeroCaM activation from the front to the rear.