BIOCHEMICAL AND METABOLIC ABNORMALITIES IN ARTICULAR-CARTILAGE FROM OSTEOARTHRITIC HUMAN HIPS .3. DISTRIBUTION AND METABOLISM OF AMINO SUGAR-CONTAINING MACROMOLECULES
BIOCHEMICAL AND METABOLIC ABNORMALITIES IN ARTICULAR-CARTILAGE FROM OSTEOARTHRITIC HUMAN HIPS .3. DISTRIBUTION AND METABOLISM OF AMINO SUGAR-CONTAINING MACROMOLECULES
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DOI：
10.2106/00004623-198163010-00017
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发表时间：
1981-01-01
影响因子：
5.3
通讯作者：
LIPPIELLO, L
中科院分区：
文献类型：
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作者：
MANKIN, HJ;JOHNSON, ME;LIPPIELLO, L
大量研究支持骨关节炎中关节软骨细胞的合成活性增加的论点，但最近的一些报告挑战了这一概念。为了阐明这个问题并进一步确定这种合成增加的产物，进行了3个实验，其中通过生物化学分析和3 H-葡糖胺和35 SO 4掺入的研究来评估人股骨头正常和骨关节炎软骨中含氨基糖大分子的合成分布和速率。证实了先前注意到的骨关节炎组织中氨基己糖含量的显著降低。这种降低主要是由于葡萄糖胺浓度的减少，并与疾病过程的严重程度呈负相关（通过先前描述的组织学-组织化学分级系统测量）。代谢研究表明，3 H-葡萄糖胺掺入软骨的葡萄糖胺和半乳糖胺组分的速率显著增加。增加的合成以非线性方式与疾病的严重程度直接相关。在正常和骨关节炎样品中，3 H-葡糖胺掺入到葡糖胺组分中的速率与其掺入到半乳糖胺组分中的速率的比率是相同的，这表明葡糖胺浓度的下降与合成活性的定性改变无关。
Numerous studies support the thesis that the synthetic activity of articular chondrocytes is increased in osteoarthritis, but several recent reports challenged this concept. To clarify this problem and to define further the products of this increased synthesis, 3 experiments were performed in which the distribution and rates of synthesis of amino sugar-containing macromolecules in normal and osteoarthritic cartilage from the human femoral head were assessed by biochemical analysis and studies of the incorporation of 3H-glucosamine and 35SO4. The previously noted significant decrease in hexosamine content in osteoarthritic tissue was demonstrated. This decrease was principally due to a diminution in glucosamine concentration and correlated inversely with the severity of the disease process (as measured by a previously described histological-histochemical grading system). Metabolic studies showed a marked increment in the rates of incorporation of 3H-glucosamine into both the glucosamine and the galactosamine fractions of the cartilage. The increased synthesis correlated directly in a non-linear fashion with the severity of the disease. The ratio of the rate of incorporation of 3H-glucosamine into the glucosamine fraction to the rate of its incorporation into the galactosamine fraction was the same in normal and osteoarthritic samples, suggesting that the decline in glucosamine concentration was not related to a qualitative alteration of synthetic activity.