Dual Transport Properties of Anion Exchanger 1 THE SAME TRANSMEMBRANE SEGMENT IS INVOLVED IN ANION EXCHANGE AND IN A CATION LEAK
Dual Transport Properties of Anion Exchanger 1 THE SAME TRANSMEMBRANE SEGMENT IS INVOLVED IN ANION EXCHANGE AND IN A CATION LEAK
复制标题
DOI：
10.1074/jbc.m110.166819
复制
发表时间：
2011-03-18
影响因子：
4.8
通讯作者：
Guizouarn, Helene
中科院分区：
文献类型：
--
作者：
Barneaud-Rocca, Damien;Borgese, Franck;Guizouarn, Helene
先前的结果表明，在人类阴离子交换器1（AE1）的特定点突变转换成一价阳离子电导的电中性阴离子交换器。在本研究中，运输网站的阴离子交换和阳离子泄漏已经研究了半胱氨酸扫描诱变和巯基试剂化学。此外，AE1所属的SLC4家族成员中的一些高度保守的氨基酸在AE1转运特性中的作用已被评估。结果表明，相同的运输网站内的AE1跨越域参与阴离子交换或阳离子运输。根据研究的AE1不同点突变的转运特性，提出了该转运位点的作用机制。
Previous results suggested that specific point mutations in human anion exchanger 1 (AE1) convert the electroneutral anion exchanger into a monovalent cation conductance. In the present study, the transport site for anion exchange and for the cation leak has been studied by cysteine scanning mutagenesis and sulfhydryl reagent chemistry. Moreover, the role of some highly conserved amino acids within members of the SLC4 family to which AE1 belongs has been assessed in AE1 transport properties. The results suggest that the same transport site within the AE1 spanning domain is involved in anion exchange or in cation transport. A functioning mechanism for this transport site is proposed according to transport properties of the different studied point mutations of AE1.