Adenosine activates mesangial cell proliferation
Adenosine activates mesangial cell proliferation
复制标题
DOI：
10.1016/s0898-6568(96)00091-5
复制
发表时间：
1997-01-01
影响因子：
4.8
通讯作者：
LopezNovoa, JM
中科院分区：
文献类型：
--
作者：
MacLaughlin, M;MartinezSalgado, C;LopezNovoa, JM
本研究探讨腺苷（ADO）对培养的系膜细胞的增殖作用，以及A（1）和/或A（2）受体在ADO增殖作用中的可能介导作用。相对于静止细胞，ADO（10（-5）M）诱导[H-3]胸苷掺入DNA的显著增加。这种增加与ADO A（1）受体激动剂R-PIA（10（-5）M）和ADO A（2）受体激动剂NECA（10（-5）M）获得的结果相似。ADO受体抑制剂茶碱（10（-4）M）可完全抑制ADO诱导的细胞增殖。NECA + A（2）受体拮抗剂PD 116，948（AT（1），10（-6）M）和PIA + A（2）受体拮抗剂PD 115，199（AT（2），10（-2）M）的组合与对照条件下维持的细胞相比未诱导任何显著差异。这些发现证明了ADO在培养的系膜细胞中的增殖作用，并且这种作用不是A（1）或A（2）受体活化所特有的。版权所有（C）1997 Elsevier Science Inc.
This study investigates the proliferative effect of adenosine (ADO) in cultured mesangial cells, and the possible mediation of A(1) and/or A(2) receptors in this proliferative effect of ADO. ADO (10(-5) M) induced a significant increase in the [H-3]thymidine incorporation into DNA with respect to quiescent cells. This increase was similar to that obtained with the ADO A(1) receptor agonist, R-PIA (10(-5) M), and with the ADO A(2) receptor agonist, NECA (10(-5) M). Theophylline (10(-4) M), an ADO receptors inhibitor, completely inhibits the ADO-induced proliferation. The combinations NECA+A(2) receptor antagonist, PD 116,948 (AT(1), 10(-6) M) and PIA + A(2) receptor antagonist, PD 115,199 (AT(2), 10(-2) M) did not induce any significant difference with respect to cells maintained in control conditions. These findings demonstrate the proliferative effect of ADO in cultured mesangial cells, and that this effect is not specific to either of A(1) or A(2) receptors activation. Copyright (C) 1997 Elsevier Science Inc.