A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae
A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae
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DOI：
10.1093/nar/gki959
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发表时间：
2005-01-01
影响因子：
14.9
通讯作者：
Dutreix, M
中科院分区：
文献类型：
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作者：
Mercier, G;Berthault, N;Dutreix, M
真核细胞通过抑制细胞周期和调节基因表达来应对DNA损伤，以确保有效的DNA修复。我们利用全球转录组分析研究了倍性和交配型在诱导不同酿酒酵母菌株对损伤反应中的作用。我们观察到单倍体菌株对DNA损伤的特异性反应似乎是由染色质调节蛋白控制的。与这些微阵列数据一致，我们发现交配型因子控制着一个报告基因的染色质依赖性沉默。这两种分析都表明，菌株（单倍体或二倍体）在只有一种交配型因子的情况下存在辐照特异性反应。这种反应取决于Hdf1和Sir2的活性。总的来说，我们的研究结果表明，存在一种依赖于交配型因子、染色质结构重塑、Sir2和Hdf1、独立于Mec1激酶的新的调控途径。
Eukaryotic cells respond to DNA damage by arresting the cell cycle and modulating gene expression to ensure efficient DNA repair. We used global transcriptome analysis to investigate the role of ploidy and mating-type in inducing the response to damage in various Saccharomyces cerevisiae strains. We observed a response to DNA damage specific to haploid strains that seemed to be controlled by chromatin regulatory proteins. Consistent with these microarray data, we found that mating-type factors controlled the chromatin-dependent silencing of a reporter gene. Both these analyses demonstrate the existence of an irradiation-specific response in strains (haploid or diploid) with only one mating-type factor. This response depends on the activities of Hdf1 and Sir2. Overall, our results suggest the existence of a new regulation pathway dependent on mating-type factors, chromatin structure remodeling, Sir2 and Hdf1 and independent of Mec1 kinase.