Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities
Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities
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DOI：
10.1016/j.aquaculture.2008.10.046
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发表时间：
2009-02
期刊：
影响因子：
4.5
通讯作者：
Xuxia Zhou;Yanbo Wang;Wei-fen Li
中科院分区：
文献类型：
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作者：
Xuxia Zhou;Yanbo Wang;Wei-fen Li
益生菌B.以南美白对虾（Penaeus vannamei）幼虾为试验对象，研究了不同浓度的凝结多糖SC 8168对幼虾个体发育阶段（Z3、M3、PL 1 - 2和PL 7 -8）的水质、存活率和消化酶活性的影响。每个处理组和对照组使用12个罐，重复3次。处理包括初始浓度为1.0× 105 cfu ml−1（T-1）、5.0× 105 cfu ml−1（T-2）和1.0× 106 cfu ml−1（T-3）的三个SC 8168水平和一个对照（不含任何益生菌），每天进行。与对照组相比，添加益生菌显著提高了所有处理组的存活率（P<0.05）。然而，在T-2和T-3之间没有发现显著差异。在幼虾早期（Z3和M3），益生菌处理组和对照组的蛋白酶活性差异不显著。在随后的个体发育阶段（PL 1 - 2和PL 7 -8），T-2的蛋白酶活性最高，与对照组相比差异显著（P<0.05）。PL 7 - 8期T-3组与PL 7 - 8期T-3组比较，差异有统计学意义（P<0.05）。在Z3、M3、PL 1 - 2和PL 7 - 8期，T-2期的淀粉酶活性显著高于对照（P<0.05）。除M3期外，T-3期淀粉酶活性均显著高于对照（P<0.05）。至于脂肪酶活性，除初始阶段（Z3）外，用SC 8168处理的组与对照组相比显示出显著差异（P<0.05）。然而，在本研究中未观察到T-1、T-2和T-3中益生菌菌株的浓度响应。这项研究的结果表明，B。一定浓度的凝结多糖SC 8168可显著提高凡纳滨对虾幼体的存活率和部分消化酶活性。
The effect of probiotic, B. coagulans SC8168, as water additive on larvae shrimp (Penaeus vannamei) based on water quality, survival rate and digestive enzyme activities was investigated at ontogenetic stages (Z3, M3, PL1–2and PL7–8). Twelve tanks with three replicates for each treatment group and control group were used. The treatments consisted of three SC8168 levels at an initial concentration of 1.0×105cfu ml−1(T-1), 5.0×105cfu ml−1(T-2) and 1.0×106cfu ml−1(T-3) and one control (without any probiotic), and were conducted every day. Addition of the probiotic significantly increased survival rate (P<0.05) for all treatments over controls. However, no significant difference was found between T-2 and T-3. At early larval stages (Z3and M3), protease activity in shrimp was not significantly different among probiotic treatments and control. At the subsequent ontogenetic stages (PL1–2and PL7–8), the highest protease activity was observed in T-2 and there was a significant difference (P<0.05) between the treatment and the control. Similar results were observed in T-3 at PL7–8stage (P<0.05). Amylase activity in T-2 at Z3, M3, PL1–2and PL7–8stages was significantly higher (P<0.05) than that in the control. The amylase activity was also increased significantly (P<0.05) in T-3 than the control except the M3stage. As for the lipase activity, assays showed a significant difference (P<0.05) in groups treated with SC8168 as compared with the control except the initial stage (Z3). However, a concentration response of probiotic strains in T-1, T-2 and T-3 was not observed in the present research. The results from this study suggest that B. coagulans SC8168 supplemented at a certain concentration could significantly increase survival rate and some digestive enzyme activities of P. vannamei larvae.