Triggering Receptor in Myeloid Cells (TREM-1) Specific Expression in Peripheral Blood Mononuclear Cells of Sepsis Patients with Acute Cholangitis
Triggering Receptor in Myeloid Cells (TREM-1) Specific Expression in Peripheral Blood Mononuclear Cells of Sepsis Patients with Acute Cholangitis
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DOI：
10.1007/s10753-009-9119-1
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发表时间：
2009-06-01
期刊：
影响因子：
5.1
通讯作者：
Gong, Jianping
中科院分区：
文献类型：
--
作者：
Liao, Rui;Liu, Zuojin;Gong, Jianping
为了确定其与急性胆管炎（AC）的关系，我们试图量化AC脓毒症患者外周血单核细胞（PBMC）中髓样细胞上表达的触发受体（TREM-1）的表达。收集2008年1 ~ 9月42例AC患者和48例重症AC（ACST）患者的外周血，采用RT-PCR检测TREM-1 mRNA，免疫细胞化学和Western blotting检测TREM-1蛋白表达。分别采用ELISA法和免疫比浊法检测血清TNF-α和C-反应蛋白的变化。ACST组TREM-1表达高于AC组（P < 0.01）。免疫组化法检测两组患者术前单核细胞TREM-1均为阳性，术后48 h阳性表达率下降。与健康对照组相比，脓毒症合并AC患者TREM-1蛋白表达水平上调。TREM-1在AC或ACST脓毒症患者中的表达具有高度的敏感性和特异性。TREM-1在AC患者PBMC中表达上调，并且比其他临床炎症标志物具有更高的敏感性和特异性，表明其在AC诱导的脓毒症中的重要性。
To determine its relationship with acute cholangitis (AC), we sought to quantify expression of triggering receptor expressed on myeloid cells (TREM-1) in peripheral blood mononuclear cells (PBMC) of sepsis patients with AC. Peripheral blood samples of 42 AC patients and 48 patients with AC of severe type (ACST) were collected from January to September, 2008 and tested for TREM-1 mRNA by RT-PCR and protein expression by immunocytochemistry and Western blotting. ELISA and immunoturbidimetry were employed to detect the changes of TNF-alpha or C-reactive protein in the serum respectively. TREM-1 expression was higher in ACST group than in AC group (P < 0.01). TREM-1 was positive in mononuclear cells by immunochemistry in both groups before operative therapy, but the positive expression rate decreased at 48 h postoperatively. Compared with healthy controls, TREM-1 protein expression levels were up-regulated in sepsis patients with AC. TREM-1 expression has highly sensitivity and specificity in sepsis patients with AC or ACST. TREM-1 is up-regulated in PBMC of AC patients, and has higher sensitivity and specificity than other clinical inflammation markers, suggesting its importance in AC-induced sepsis.