Replicative Senescence in Human Fibroblasts Is Delayed by Hydrogen Sulfide in a NAMPT/SIRT1 Dependent Manner.
Replicative Senescence in Human Fibroblasts Is Delayed by Hydrogen Sulfide in a NAMPT/SIRT1 Dependent Manner.
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DOI：
10.1371/journal.pone.0164710
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Tabibzadeh S
中科院分区：
文献类型：
--
作者：
Sanokawa-Akakura R;Akakura S;Tabibzadeh S
最近的证据表明，硫化氢具有细胞保护和延缓衰老的作用。然而，这些特性的机制还没有完全被理解。在这里，我们发现在复制衰老的成人皮肤成纤维细胞中，主要的硫化氢产生酶CBS的表达和硫化氢的产生被协调地减少。H_2S产生的减少与复制衰老的特征出现的时间段相同，包括SA-β-Gal的积累，p16、p21和RRM2B的表达增强，而RRM2、hTERT、SIRT1、NAMPT和NAD/NADH的表达均下降。外源H_2S可增加hTERT、NAMPT、SIRT1的表达和NAD/NADH比值。此外，硫化氢以NAMPT和SIRT1依赖的方式保护端粒酶逆转录酶的表达，并延缓复制衰老的开始，这体现在与AGE相关的SA-β-Gal的积累减少和增殖停止。延缓细胞增殖能力的丧失而不存在诱变风险，这表明使用硫化氢可以延缓生物体衰老的不利影响。
Recent evidence suggests that hydrogen sulfide (H2S) has cytoprotective and anti-aging effects. However, the mechanisms for such properties are not fully understood. Here, we show that the expression of the main H2S producing enzyme, CBS, and production of H2S are coordinately diminished in replicative senescent adult human dermal fibroblasts. The reduced production of H2S falls within the same time-frame that the hallmarks of replicative senescence appear including accumulation of SA–β-Gal, enhanced expression of p16, p21, and RRM2B while the expression of RRM2, hTERT, SIRT1, NAMPT, and NAD/NADH ratio all fall. Exogenous H2S increases the expression of hTERT, NAMPT, SIRT1 and NAD/NADH ratio in treated cells. Moreover, H2S safeguards the expression of hTERT in a NAMPT and SIRT1 dependent manner and delays the onset of replicative senescence as evidenced by reduced accumulation of age associated SA–β-Gal and cessation of proliferation. Postponement of loss of cell proliferative capacity without risk of mutagenesis shows implications for use of H2S in delaying the adverse effects of senescence in organisms.
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DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
14.9
作者：
SCHREIBER, E;HARSHMAN, K;FONTANA, A
通讯作者：
FONTANA, A
影响因子：
2.8
作者：
Greenwood, SK;Hill, RB;Galloway, SM
通讯作者：
Galloway, SM
Sirt1 使 c-Myc 去乙酰化并促进 c-Myc/Max 结合
DOI：
10.1016/j.biocel.2011.07.006
发表时间：
2011-11-01
影响因子：
4
作者：
Mao, Beibei;Zhao, Guowei;Liang, Chih-Chuan
通讯作者：
Liang, Chih-Chuan
影响因子：
3
作者：
Imai, Shin-ichiro
通讯作者：
Imai, Shin-ichiro