Genome-Wide Identification, Characterization and Expression Analysis of Xyloglucan Endotransglucosylase/Hydrolase Genes Family in Barley (Hordeum vulgare)
Genome-Wide Identification, Characterization and Expression Analysis of Xyloglucan Endotransglucosylase/Hydrolase Genes Family in Barley (Hordeum vulgare)
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DOI：
10.3390/molecules24101935
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发表时间：
2019-05-02
期刊：
影响因子：
4.6
通讯作者：
Wu, Feibo
中科院分区：
文献类型：
--
作者：
Fu, Man-Man;Liu, Chen;Wu, Feibo
木葡聚糖内切转葡糖基酶/水解酶（XTH）是木葡聚糖修饰酶的一个家族，在木葡聚糖交联的构建和重组中起着重要作用。然而，还没有对大麦中的该基因家族进行全面的研究。利用最近完成的大麦基因组数据库，共鉴定出24个HvXTH基因（命名为HvXTH 1 -24）和一个EG 16成员。系统进化分析表明，24个HvXTH基因可分为I/II、III-A和III-B 3个系统进化群，HvXTH 15属于祖先群。除HvXTH 15外，所有HvXTH蛋白均具有保守的N-糖基化位点。HvXTH基因在大麦染色体上的定位结果表明，这24个基因在7条染色体上分布不均匀，其中10个基因特异性地定位在7 H染色体上。基于结构的序列比对表明，每个XTH具有高度保守的结构域（ExDxE）负责催化活性。基于大麦基因组数据库的表达谱显示，HvXTH家族成员在不同组织和不同时期显示不同的表达模式。本研究首次对大麦HvXTH基因家族进行了系统的基因组分析。我们的研究结果为阐明HvXTH基因在大麦生长发育中的作用提供了有价值的信息。
Xyloglucan endotransglucosylase/hydrolases (XTHs)a family of xyloglucan modifying enzymesplay an essential role in the construction and restructuring of xyloglucan cross-links. However, no comprehensive study has been performed on this gene family in barley. A total of 24 HvXTH genes (named HvXTH1-24) and an EG16 member were identified using the recently completed genomic database of barley (Hordeum vulgare). Phylogenetic analysis showed that 24 HvXTH genes could be classified into three phylogenetic groups: (I/II, III-A and III-B) and HvXTH15 was in the ancestral group. All HvXTH protein membersexcept HvXTH15had a conserved N-glycosylation site. The genomic location of HvXTHs on barley chromosomes showed that the 24 genes are unevenly distributed on the 7 chromosomes, with 10 of them specifically located on chromosome 7H. A structure-based sequence alignment demonstrates that each XTH possesses a highly conserved domain (ExDxE) responsible for catalytic activity. Expression profiles based on the barley genome database showed that HvXTH family members display different expression patterns in different tissues and at different stages. This study is the first systematic genomic analysis of the barley HvXTH gene family. Our results provide valuable information that will help to elucidate the roles of HvXTH genes in the growth and development of barley.