Isolation of a novel tetrasaccharide, bemisiotetrose, and glycine betaine from silverleaf whitefly honeydew
Isolation of a novel tetrasaccharide, bemisiotetrose, and glycine betaine from silverleaf whitefly honeydew
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从银叶粉虱蜜露中分离新型四糖、半硫四糖和甘氨酸甜菜碱
DOI：
10.1021/jf9601170
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
R. Nieman
中科院分区：
文献类型：
--
作者：
Yuan;D. Hendrix;R. Nieman
取食棉花(Gossypium hirsutom L.)的银叶粉虱(Bomesia Argentifolii Bellowers And Perring)分泌的蜜露中的低聚糖通过凝胶渗透层析和梯度洗脱高效液相色谱柱和蒸发光散射检测器分离得到。从高效液相洗脱液中分离得到一种四糖，其结构经质谱学、甲基化分析、一维和二维核磁共振确证。这些分析的结果与一种高度对称的四糖相一致：O-α-d-glucopyranosyl-(1→4)-O-α-d-glucopyranosyl-(1↔1)-O-α-d-glucopyranosyl-(4←1)-α-d-glucopyranoside. 此外，在蜜露糖的高效液相色谱图谱中还检测到甘氨酸甜菜碱(N，N，N-三甲基甘氨酸)的显著峰。关键词：昆虫；碳水化合物；高效液相色谱法；棉花
Oligosaccharides in the honeydew secreted by the silverleaf whitefly (Bemisia argentifolii Bellows and Perring) feeding upon cotton (Gossypium hirsutum L.) were isolated by gel permeation chromatography followed by gradient elution HPLC employing an amine column and an evaporative light-scattering detector. A tetrasaccharide was collected from the HPLC eluate and characterized by mass spectral and methylation analysis and by 1D and 2D NMR. The results of these analyses are consistent with a highly symmetrical tetrasaccharide: O-α-d-glucopyranosyl-(1→4)-O-α-d-glucopyranosyl-(1↔1)-O-α-d-glucopyranosyl-(4←1)-α-d-glucopyranoside. In addition, a significant peak of glycine betaine (N,N,N-trimethylglycine) was detected in the HPLC chromatograms of sugars from this honeydew. Keywords: Insect; carbohydrate; HPLC; cotton