Accessory cell dependence of lectin‐induced proliferation of mouse T lymphocytes
Accessory cell dependence of lectin‐induced proliferation of mouse T lymphocytes
复制标题
凝集素诱导的小鼠 T 淋巴细胞增殖的辅助细胞依赖性
DOI：
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发表时间：
1977
影响因子：
5.4
通讯作者：
M. Raff
中科院分区：
文献类型：
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作者：
S. Habu;M. Raff
小鼠淋巴结细胞暴露于羰基铁和磁铁中以去除吞噬细胞，并通过Sephadex G‐10和尼龙羊毛，在塑料上孵育12小时以去除贴壁细胞及其前体。在这个巨噬细胞耗尽的群体（占起始群体的3 - 5%）中，超过99%的细胞是Thy‐1+和Ly‐1+，而少于2%的细胞是Ly‐2+。这些细胞通常不能合成可检测数量的DNA，当与豆豆蛋白A一起培养时，对植物血凝素的反应很差。少量同基因或异体腹膜细胞、纯化的腹膜巨噬细胞或来自小鼠胚胎“成纤维细胞”三级培养的细胞完全重建了这些增殖反应，但3T3细胞、P815肥大细胞瘤细胞或Nulli SCC‐1胚胎癌细胞或2‐巯基乙醇不能重建这些增殖反应。重构的腹膜细胞为Thy‐1−、Ia+，存在于nu/nu小鼠中；虽然它们必须活着才能重组，但它们不必分裂。这些结果与假设一致，即凝集素诱导的T细胞增殖与抗原诱导的T细胞增殖一样，可能涉及与主要组织相容性复合体（la）决定因素相关的刺激配体的双重识别。
Mouse lymph node cells were exposed to carbonyl iron and a magnet to remove phagocytic cells, and passed over Sephadex G‐10 and nylon wool and incubated for 12 h on plastic to remove adherent cells and their precursors. More than 99 % of the cells in this macrophage‐depleted population (which constituted 3‐5 % of the starting population) were Thy‐1+ and Ly‐1+, while less than 2 % were Ly‐2+. These cells usually did not synthesize detectable amounts of DNA when cultured with concanavalin A and responded poorly to phytohemagglutinin. These proliferative responses were completely reconstituted by small numbers of syngeneic or allogeneic peritoneal cells, purified peritoneal macrophages or cells from tertiary cultures of mouse embryo ‘fibroblasts’, but not by 3T3 cells, P815 mastocytoma cells or Nulli SCC‐1 embryonal carcinoma cells, or by 2‐mercaptoethanol. The reconstituting peritoneal cells were Thy‐1−, Ia+ and present in nu/nu mice; although they had to be alive to reconstitute, they did not have to divide. These results are consistent with the hypothesis that T cell proliferation induced by lectins, like that induced by antigens, may involve the dual recognition of stimulating ligand in association with major histocompatibility complex (la) determinants.