Angiogenesis in the New Zealand obese mouse model fed with high fat diet.
Angiogenesis in the New Zealand obese mouse model fed with high fat diet.
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DOI：
10.1186/1476-511x-8-13
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发表时间：
2009-04-06
影响因子：
4.5
通讯作者：
Dembinska-Kiec A
中科院分区：
文献类型：
--
作者：
Balwierz A;Polus A;Razny U;Wator L;Dyduch G;Tomaszewska R;Scherneck S;Joost H;Dembinska-Kiec A
肥胖及其并发症导致血管损伤、动脉粥样硬化、糖尿病和病理性血管生成。新西兰肥胖小鼠模型是研究肥胖及其相互关系的模型之一。本研究的目的是检查高脂饮食对NZO小鼠生化参数变化以及对血管生成过程的影响。用标准（ST）或高脂肪（HF）饮食喂养NZO小鼠七周。监测体重和血清生化指标。免疫组化染色观察PECAM 1阳性血管样结构，并通过芯片检测基质胶穿透细胞的基因表达（实时荧光PCR法证实）。喂食HF饲料的小鼠出现肥胖。新创建的带管腔血管数量与高血糖和动物体重增加相关。在HF饮食期间，基质胶中PECAM 1阳性细胞的数量趋于增加。微阵列结果显示基因表达的变化（氧化应激和胰岛素抵抗的激活，细胞凋亡和细胞分化的抑制），但没有内皮细胞网络成熟的标志物。在HF饮食的NZO小鼠中观察到的变化证明了高血糖症相关的血管生成激活，导致病理性不成熟网络的形成。
Obesity and its complications lead to vascular injury, atherosclerosis, diabetes and pathological angiogenesis. One of the models to study the obesity and its entanglements is the New Zealand Obese mice model. Aim of this study was to check the effect of high fat diet on changes in biochemical parameters as well as on process of angiogenesis in NZO mice. NZO mice were fed with standard (ST) or high fat (HF) diet for seven weeks. Body weight and serum biochemical parameters were monitored. The PECAM1 positive vessel-like structures immunostaining, as well as the gene expression of the matrigel penetrating cells by microarray (confirmed by real-time PCR method) were analyzed. Mice fed with HF diet developed obesity. Number of newly created vessels with lumen was correlated with hyperglycemia and animal weight gain. The number of PECAM1 positive cells in matrigel tended to increase during HF diet. Microarray results revealed changes in gene expression (activation of the oxidative stress and insulin resistance, inhibition of apoptosis and cell differentiation), however without markers of endothelial cell network maturation. Observed changes in the NZO mice on HF diet argue for the hyperglycemia related activation of angiogenesis, leading to the formation of pathological, immature network.