DISSOCIATION BETWEEN TRANSFORMED AND DIFFERENTIATED PHENOTYPE IN RAT-THYROID EPITHELIAL-CELLS AFTER TRANSFORMATION WITH A TEMPERATURE-SENSITIVE MUTANT OF THE KIRSTEN MURINE SARCOMA-VIRUS
DISSOCIATION BETWEEN TRANSFORMED AND DIFFERENTIATED PHENOTYPE IN RAT-THYROID EPITHELIAL-CELLS AFTER TRANSFORMATION WITH A TEMPERATURE-SENSITIVE MUTANT OF THE KIRSTEN MURINE SARCOMA-VIRUS
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DOI：
10.1128/mcb.3.11.2099
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
VECCHIO, G
中科院分区：
文献类型：
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作者：
COLLETTA, G;PINTO, A;VECCHIO, G
体外用Kirsten鼠肉瘤病毒的温度敏感突变体感染的分化的大鼠甲状腺上皮细胞在允许温度（33 ℃）下表达。C）转化细胞的一些典型表型特性，包括形态学特征、琼脂中的集落形成和新生动物中的肿瘤诱导。这些分化细胞的特异性功能标志物，即，甲状腺球蛋白的合成/分泌、甲状腺球蛋白mRNA的合成和碘摄取在33 ℃生长期间被阻断。C.正常形态、在琼脂中不能生长和最佳生长所需的激素在转换到不允许转化的温度（39 ℃）后全部恢复。C），尽管典型的分化功能仍然被阻断。感染白血病辅助病毒克隆（Moloney或Kirsten鼠白血病病毒）不会导致大鼠甲状腺上皮细胞分化表型的丧失，从而证明分化表型的丧失是由肉瘤病毒组分引起的。这些结果表明，转化分化的大鼠甲状腺上皮细胞的某些表型特性的表达是在p21的温度敏感活性的直接控制下，在甲状腺上皮细胞的2个典型的分化标志物的表达的阻断是不可逆的，可能是由不同的机制控制。
Differentiated rat thyroid epithelial cells, infected in vitro with a temperature-sensitive mutant of the Kirsten murine sarcoma virus, expressed at the permissive temperature (33.degree. C) some phenotypic properties typical of transformed cells, including morphological features, colony formation in agar and induction of tumors in newborn animals. Specific functional markers of these differentiated cells, i.e., synthesis/secretion of thyroglobulin, synthesis of thyroglobulin mRNA and iodide uptake, were blocked during growth at 33.degree. C. Normal morphology, failure to grow in agar and the requirement of hormones for optimal growth were all restored after shifting to the temperature nonpermissive for transformation (39.degree. C), though the typical differentiated functions remained blocked. Infection with a leukemia helper virus clone (Moloney or Kirsten murine leukemia virus) did not lead to the loss of the differentiated phenotype of rat epithelial thyroid cells, thus demonstrating that the loss of the differentiated phenotype is caused by the sarcoma virus component. These results indicate that the expression of some of the phenotypic properties of transformed differentiated rat thyroid epithelial cells is under the direct control of the p21 thermosensitive activity; the block in the expression of 2 typical differentiation markers of thyroid epithelial cells is irreversible and probably controlled by different mechanisms.