Transcripts Associated with Non‐Acclimated Freezing Response in Two Barley Cultivars
Transcripts Associated with Non‐Acclimated Freezing Response in Two Barley Cultivars
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DOI：
10.3835/plantgenome2008.06.0344
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发表时间：
2008-07
期刊：
影响因子：
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通讯作者：
B. Koo;B. Bushman;I. Mott
中科院分区：
文献类型：
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作者：
B. Koo;B. Bushman;I. Mott
与经历适应期的秋季生长的植物相比，遭受春季冰冻的植物通常没有足够长的适应时间来赋予对零下冰冻胁迫的耐受性。世界各地的大麦 (Hordeum vulgare L.) 会定期遭受春末冰冻，导致产量严重损失。我们使用 barley22K Affymetrix GeneChip 测量转录本丰度，检查了“Dicktoo”和“Keunal”大麦品种在拔节期对非驯化冷冻 (NAF) (-3°C) 的反应。 Dicktoo 对 NAF 冷冻的耐受时间比 Keunal 更长，并且在 NAF 处理下具有更多的可变微阵列探针组。根据它们在 Dicktoo 与 Keunal 中的差异反应，选择了 313 个探针组作为 NAF 的反应基因。这些探针组中有 71 个具有基于同源性的注释，其中 49 个显示与 Dicktoo 对照、Keunal 对照和 Keunal NAF 处理相比，Dicktoo NAF 中的转录水平降低，22 个显示转录水平增加。与 Keunal NAF 相比，Dicktoo NAF 的探针组中转录因子、昼夜节律相关基因和其他涉及光周期响应的基因上调，而涉及 RNA 代谢以及水和糖转运的基因则下调。 Dicktoo 和 Keunal 对 NAF 的转录组反应也不同于基于驯化的冷冻治疗，其中 43% 的 Dicktoo NAF 和 37% Keunal NAF 探针组是独特的。
Plants subjected to spring freezes are usually not acclimated sufficiently long to confer tolerance to sub‐zero freezing stress in contrast to fall‐grown plants which experience an acclimation period. Barley (Hordeum vulgare L.) periodically suffers from late spring freezes in areas throughout the world, with significant yield loss. We examined the response of ‘Dicktoo’ and ‘Keunal’ barley varieties to non‐acclimated freezing (NAF) (−3°C) at the jointing stage using the barley22K Affymetrix GeneChip to measure transcript abundances. Dicktoo was tolerant of NAF freezing for a longer period of time than Keunal, and had substantially more variable microarray probesets under the NAF treatment. Three hundred and thirteen probesets were selected as responsive genes to NAF based on their differential response in Dicktoo vs. Keunal. Seventy‐one of these probesets had homology‐based annotations, with 49 of them showing reduced transcript levels in Dicktoo NAF vs. Dicktoo control, Keunal control, and Keunal NAF treatments, and 22 showing increased transcript levels. Transcription factors, circadian related genes, and other genes involved in photoperiod response were represented among the probesets upregulated in Dicktoo NAF compared to Keunal NAF, while genes involved in RNA metabolism and water and sugar transport were downregulated. The transcriptome response of both Dicktoo and Keunal to NAF also differed from acclimation‐based freezing treatments, with 43% of Dicktoo NAF and 37% of Keunal NAF probesets unique.