Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties of Rose Essential Oil, Hydrosol and Absolute

Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties of Rose Essential Oil, Hydrosol and Absolute
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DOI:
10.1007/s00284-009-9475-y
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发表时间:
2009-11-01
影响因子:
2.6
通讯作者:
Secilmis-Canbay, Hale
Secilmis-Canbay, Hale
中科院分区:
生物学4区
文献类型:
--
作者:
Ulusoy, Seyhan;Bosgelmez-Tinaz, Guelguen;Secilmis-Canbay, Hale

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研究了野蔷薇的抗氧化活性、抑菌活性和总酚含量。研究了花提取物(净油、精油和水溶胶)。用GC-MS对提取物的化学成分进行分析,发现玫瑰净油的主要成分为苯乙醇(78.38%),玫瑰精油和水溶胶的主要成分为香茅醇和香叶醇(>55%)。生育酚和胡萝卜素水平通过高效液相色谱(HPLC)分析测定。发现玫瑰净油的β-胡萝卜素(422.3 +/- 35.6 ppm)、α-生育酚(2397.1 +/- 72.5 ppm)和γ-生育酚(343.1 +/- 28.4 ppm)水平高于精油和水溶胶。同时测定了它们的总酚含量。总酚含量的测试提取物变化从5.2到2134.3 GAE/ mg L-1。玫瑰净油和精油含有高水平的酚类物质,并对大肠杆菌(ATCC 25922)、铜绿假单胞菌(ATCC 27853)、枯草芽孢杆菌(ATCC 6633)、金黄色葡萄球菌(ATCC 6538)、紫色色杆菌(ATCC 12472)和胡萝卜软腐欧文氏菌(ATCC 39048)菌株表现出较强的抗菌活性。
The antioxidant and antibacterial activities, and total phenolic contents of Rosa damascena Mill. flower extracts (absolute, essential oil and hydrosol) were investigated. The chemical compositions of these extracts were analysed by GC-MS. Phenylethyl alcohol (78.38%) was found to be the main constituent of rose absolute, while citrenellol and geraniol were the major compounds (>55%) of rose essential oil and hydrosol. Tocopherol and carotene levels were determined by high performance liquid chromatography (HPLC) analysis. The levels of beta carotene (422.3 +/- 35.6 ppm), alpha tocopherol (2397.1 +/- 72.5 ppm) and gamma tocopherol (343.1 +/- 28.4 ppm) of rose absolute were found to be higher than that of essential oil and hydrosol. Their total phenolic contents were also evaluated. The total phenolic content of the tested extracts varied from 5.2 to 2134.3 GAE/ mg L-1. Rose absolute and essential oil contained high levels of phenolics and demonstrated strong antibacterial activity against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), Chromobacterium violaceum (ATCC 12472) and Erwinia carotovora (ATCC 39048) strains.