CYTOGENETICS IN NUTRITIONAL MEGALOBLASTIC-ANEMIA - PROLONGED PERSISTENCE OF CHROMOSOMAL-ABNORMALITIES IN LYMPHOCYTES AFTER REMISSION
CYTOGENETICS IN NUTRITIONAL MEGALOBLASTIC-ANEMIA - PROLONGED PERSISTENCE OF CHROMOSOMAL-ABNORMALITIES IN LYMPHOCYTES AFTER REMISSION
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DOI：
10.1159/000206040
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发表时间：
1986-01-01
影响因子：
2.4
通讯作者：
GAREWAL, G
中科院分区：
文献类型：
--
作者：
DAS, KC;MOHANTY, D;GAREWAL, G
染色体研究进行了植物血凝素刺激的淋巴细胞培养物和骨髓细胞没有文化的115例巨幼细胞性贫血造成的营养缺乏叶酸和维生素B12。在两个细胞系中观察到基本相似的染色体异常。其特征在于引人注目的形态畸变，如伸长和去螺旋化（解螺旋或不完全收缩），染色体断裂和着丝粒扩展的频率增加。数值异常，染色单体交换和易位几乎不存在。放射自显影研究后脉冲标记3 H-胸腺嘧啶核苷在终端6小时的植物血凝素刺激的淋巴细胞培养的染色体显示的差异模式的放射性分布在巨幼细胞淋巴细胞的染色体相比，从正常的淋巴细胞。在开始使用缺乏维生素治疗后的不同时间间隔，对其中65名患者进行了重复的染色体研究。治疗48小时后，骨髓中的染色体异常显著减少;但这些形态学染色体变化（即去螺旋化和断裂）中的许多在相当大比例的患者的淋巴细胞中持续存在，持续时间可变，直至缺乏维生素治疗导致血液学缓解后6-12个月。然而，这些异常在反复感染的患者缓解后并不持续。
Chromosomal studies were performed in phytohaemagglutinin-stimulated cultures of lymphocytes and in bone marrow cells without culture from 115 patients with megaloblastic anaemia resulting from nutritional deficiency of folate and vitamin B12. Essentially similar chromosomal abnormalities were observed in the two cell lines. These were characterized by striking morphological aberrations such as elongation and despiralization (uncoiling or incomplete contraction), increased frequency of chromosome breakage and centromere spreading. Numerical abnormalities, chromatid exchanges and translocations were virtually absent. Autoradiographic studies of chromosomes after pulse-labelling with 3H-thymidine during the terminal 6 h of phytohaemagglutinin-stimulated lymphocyte cultures revealed a differential pattern of distribution of the radionucleotide in the chromosomes of megaloblastic lymphocytes as compared to those from normal lymphocytes. Repeated chromosomal studies were done in 65 of these patients at various intervals after starting therapy with the deficient vitamins. After 48 h of therapy, chromosomal abnormalities were remarkably reduced in the bone marrow; but many of these morphological chromosomal changes (i.e. despiralization and breaks) persisted in the lymphocytes of a significant proportion of patients for variable periods up to 6-12 months after haematological remission resulting from therapy with the deficient vitamins. These abnormalities did not, however, persist after remission in those patients who had repeated episodes of infections.