Liquid Crystal Emulsions as the Basis of Biological Sensors for the Optical Detection of Bacteria and Viruses
Liquid Crystal Emulsions as the Basis of Biological Sensors for the Optical Detection of Bacteria and Viruses
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DOI：
10.1002/adfm.200900399
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发表时间：
2009-07-24
影响因子：
19
通讯作者：
Caruso, Frank
中科院分区：
文献类型：
--
作者：
Sivakumar, Sri;Wark, Kim L.;Caruso, Frank
一种基于单分散液晶(LC)乳滴的多功能传感方法可以检测和区分不同类型的细菌(Gram+ve和-ve)和病毒(有包膜和无包膜)。4-氰基-4‘-戊基联苯在与革兰氏菌(E.Coli)和脂膜病毒(A/NWS/Tokyo/67)接触时，从双极构型转变为放射状构型。这种转变与脂质从生物体转移到微米级液滴的界面上是一致的。相反，在革兰氏+VE细菌(枯草芽孢杆菌和微球菌)的存在下，没有观察到向放射状构型的转变。黄体)和无包膜病毒(M13辅助噬菌体)。该液滴可检测到少量(1-5)和低浓度(10(4)Pfiu mL(-1))的A/NWS/Tokyo/67病毒。与磷脂脂质体(类似于大肠杆菌细胞壁脂)孵育的单分散LC乳剂显示，取向变化是在每个脂分子的面积类似于LC液滴上的46埃(2)(类似于每个液滴的1.6×10(8)脂分子)触发的。这种方法代表了一种新的方法，可以根据细菌和病毒的细胞壁/包膜结构来感知和区分它们，为开发一类新型的基于LC微滴的生物传感器铺平了道路。
A versatile sensing method based on monodisperse liquid crystal (LC) emulsion droplets detects and distinguishes between different types of bacteria (Gram +ve and -ve) and viruses (enveloped and non-enveloped). LCs of 4-cyano-4'-pentylbiphenyl transition from a bipolar to radial configuration when in contact with Cram -ve bacteria (E. coli) and lipid-enveloped viruses (A/NWS/Tokyo/67). This transition is consistent with the transfer of lipid from the organisms to the interfaces of the micrometer-sized LC droplets. In contrast, a transition to the radial configuration is not observed in the presence of Gram +ve bacteria (Bacillus subtilis and Micrococcus. luteus) and non-enveloped viruses (M 13 helper phage). The LC droplets can detect small numbers of E. coli bacteria (1-5) and low concentrations (10(4) Pfiu mL(-1)) of A/NWS/Tokyo/67 virus. Monodisperse LC emulsions incubated with phosholipid liposomes (similar to the E coli cell wall lipid) reveal that the orientational change is triggered at an area per lipid molecule of similar to 46 angstrom(2) on an LC droplet (similar to 1.6 x 10(8) lipid molecules per droplet). This approach represents a novel means to sense and differentiate between types of bacteria and viruses based on their cell-wall/envelope structure, paving the way for the development of a new class of LC microdroplet-based biological sensors.