MLH1-focus mapping in birds shows equal recombination between sexes and diversity of crossover patterns
MLH1-focus mapping in birds shows equal recombination between sexes and diversity of crossover patterns
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DOI：
10.1007/s10577-006-1059-0
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发表时间：
2006-09-01
影响因子：
2.6
通讯作者：
Pigozzi, M. I.
中科院分区：
文献类型：
--
作者：
Calderon, P. L.;Pigozzi, M. I.
使用的错配修复蛋白MLH1在卵母细胞和精母细胞的日本鹌鹑和斑胸草雀的免疫定位，我们估计的平均金额的重组在每一个性别的两个物种。在每种情况下，MLH1病灶的数量在雄性和雌性中是统计学上相等的，并且由此产生的性别平均图长度在日本鹌鹑中为2800 cM，在斑胸草雀中为2275 cM。在日本鹌鹑中，MLH 1病灶沿着大二价体有规律地分布，每Mb对的重组率略低于鸡。在斑胸草雀的MLH1焦点的macrobivalents大幅减少的数量相对于日本鹌鹑，他们表现出显着的本地化在两种性别。有人提出，缺乏性别依赖的重组差异可能是一个扩展的功能之间的鸟类和不同的重组模式，这里观察到的交叉反映了不同的控制，尽管关于核型不对称性和DNA含量的相似性。我们讨论了鸟类和哺乳动物之间差异的可能原因，这些差异表现出性别依赖的重组差异。
Using immunolocalization of the mismatch-repair protein MLH1 in oocytes and spermatocytes of the Japanese quail and the zebra finch, we estimated the average amount of recombination in each sex of both species. In each case the number of MLH1 foci is statistically equivalent in males and females and the resulting sex-averaged map lengths are 2800 cM in the Japanese quail and 2275 cM in the zebra finch. In the Japanese quail the MLH1 foci are regularly distributed along the macrobivalents and recombination rates per Mb pair are somewhat lower compared to the chicken. In the zebra finch the MLH1 foci on the macrobivalents are substantially reduced in number relative to the Japanese quail and they show remarkable localization in both sexes. It is proposed that the lack of sex-dependent differences in recombination might be an extended feature among birds and that the different recombination patterns observed here reflect different controls of crossing over in spite of similarities regarding karyotypic asymmetry and DNA content. We discussed possible causes of the differences between birds and mammals, which show sex-dependent recombination differences.