Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant
Reduced gibberellin response affects ethylene biosynthesis and responsiveness in the Arabidopsis gai eto2-1 double mutant
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DOI：
10.1111/j.1469-8137.2007.02263.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Van Der Straeten, Dominique
中科院分区：
文献类型：
--
作者：
De Grauwe, Liesbeth;Chaerle, Laury;Van Der Straeten, Dominique
乙烯和赤霉素（GAs）控制着植物相似的发育过程。乙烯的作用至少部分是调节DELLA蛋白的积累，DELLA蛋白是植物生长的关键调节因子，其抑制GA反应。为了扩展我们对乙烯-GA串扰的认识，并揭示乙烯和GA途径的调节如何影响全局植物生长，赤霉素不敏感（gai），乙烯过量产生2-1（eto 2 -1）双突变体，其具有降低的GA信号传导（由gai引起）和增加的乙烯生物合成（由eto 2 -1引起）。双突变体表现出协同反应，在根和芽的生长，在诱导花的转变，并在花序长度，表明这两个途径之间的串扰发生在不同的植物器官在整个发展。此外，乙烯-GA相互作用的改变影响根冠通讯，证明了在双突变体的增强芽：根比。与单突变体和野生型相比，双突变体在幼苗期和莲座期都增加了活性GA（4）的含量，并且与gai突变体不同，它们对GA处理敏感。最后，研究表明，双突变体的协同反应不是由乙烯生物合成增加引起的，而是在光照下，对乙烯敏感性的增强可能，至少部分是，对观察到的表型负责。
Ethylene and gibberellins (GAs) control similar developmental processes in plants. The role of ethylene is at least in part to regulate the accumulation of DELLA proteins, key regulators of plant growth, which suppress the GA response.To expand our knowledge of ethylene-GA crosstalk and to reveal how the modulation of the ethylene and GA pathways affects global plant growth, the gibberellin-insensitive (gai), ethylene-overproducing 2-1 (eto2-1) double mutant, which has decreased GA signalling (resulting from gai) and increased ethylene biosynthesis (resulting from eto2-1), was characterized.Both single mutations resulted in reduced elongation growth. The double mutant showed synergistic responses in root and shoot growth, in induction of floral transition, and in inflorescence length, showing that crosstalk between the two pathways occurs in different plant organs throughout development. Furthermore, the altered ethylene-GA interactions affected root-shoot communication, as evidenced by an enhanced shoot:root ratio in the double mutant. When compared with both single mutants and the wild type, double mutants had enhanced content of active GA(4) at both the seedling and the rosette stages, and, unlike the gai mutant, they were sensitive to GA treatment.Finally, it was shown that synergistic responses in the double mutant were not caused by elevated ethylene biosynthesis but that, in the light, enhanced sensitivity to ethylene may, at least in part, be responsible for the observed phenotype.