Gallus gallus aggrecan gene-based phylogenetic analysis of selected avian taxonomic groups
Gallus gallus aggrecan gene-based phylogenetic analysis of selected avian taxonomic groups
复制标题
DOI：
10.1007/s10709-004-5184-4
复制
发表时间：
2005-05-01
期刊：
影响因子：
1.5
通讯作者：
Tu, ZJ
中科院分区：
文献类型：
--
作者：
Smith, EJ;Shi, L;Tu, ZJ
线粒体DNA(MtDNA)序列仍然是鸟类系统发育分析中最广泛使用的序列。然而，线粒体DNA序列的一个主要限制是线粒体基因作为一个单一的连锁群遗传。在这里，我们描述了一个540个碱基的DNA序列，该序列对应于鸡核集聚蛋白基因(Agc1)的G3结构域，用于雀形目的主要类群的系统发育分析，包括雀形科、努米迪科和齿形科。我们还包括了一些被一些人认为是克莱亚科的物种和另一些人认为的被认为是雀形科的物种。未校正的G3片段的序列差异范围从松鸡之间的1%到鹰形目中一些遥远群体之间的36%。这些序列含有39-48%的AT核苷酸，大多数比较的转换/颠换比率都在1.5以上。采用最大简约距离算法和Bootstrap分析方法，以鹰形目Oxyura jamaicensis的G3序列为外类群，构建系统发育树。这些树与用禽肉瘤和白血病病毒Gag基因描述的树以及从血红蛋白和溶菌酶c的氨基酸序列描述的树是一致的。我们的数据也支持用mtDNA序列定义的雀形目之间的关系。除了五个主要家族的普遍支持外，我们的数据也与以前的工作相一致，表明非洲斑潜蝇和家鸡存在。Tetraoninae是Phasianidae中一个有良好支持的单系亚科。这些结果表明，AGC1序列符合新的核DNA序列的标准，可以用来帮助解决雀形目之间的关系。
Mitochondrial DNA (mtDNA) sequences remain the most widely used for phylogenetic analysis in birds. A major limitation of mtDNA sequences, however, is that mitochondria genes are inherited as a single linkage group. Here we describe the use of a 540-bp DNA sequence corresponding to the G3 domain of Gallus gallus nuclear aggrecan gene (AGC1) for phylogenetic analysis of the main groups of Galliformes including Phasianidae, Numididae, and Odontophoridae. We also included species from Cracidae and Megapodiidae which are considered by some as Craciformes and others, including here as Galliformes. The uncorrected sequence divergence of the G3 fragments ranges from 1% among the grouses to 36% between some of the distant groups within Galliformes. These sequences contain 39-48% AT nucleotides and the ratios of transition versus transversion are above 1.5 in majority of the comparisons. Using G3 sequences from an Anseriform, Oxyura jamaicensis, as out-groups, phylogenetic trees were obtained using maximum parsimony and distance algorithms and bootstrap analyses. These trees were consistent with those described using Avian sarcoma and leucosis virus gag genes and those from amino acid sequences of hemoglobin and lysozyme c. Our data also support relationships among Galliformes which were defined using mtDNA sequences. In addition to the general support of the five main families of Galliformes, our data are also consistent with previous work that showed Francolinus africanus and Gallus gallus are in the. same clade and that Tetraoninae is a well-supported monophyletic subfamily within Phasianidae. The results presented here suggest that the AGC1 sequences meet the criterion of novel nuclear DNA sequences that can be used to help resolve the relationships among Galliformes.