The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens
The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens
复制标题
巨噬细胞在 T 辅助细胞生成中的作用。
DOI：
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发表时间：
1975
影响因子：
15.3
通讯作者：
Marc Feldmann
中科院分区：
文献类型：
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作者：
Peter Erb;Marc Feldmann
体外辅助细胞诱导非颗粒抗原需要T细胞和巨噬细胞的合作，但如果巨噬细胞是同种异体的，则不会发生。对此原因进行了调查。同种异体巨噬细胞的功能障碍被排除与它们的同源T细胞的培养物;抑制细胞诱导被排除在混合物实验。因此，T细胞和巨噬细胞只有在基因相似的情况下才能合作。对所涉及的遗传位点进行了定位。使用同类系不同的H-2复合物，T-巨噬细胞相互作用的遗传控制定位在H- 2区域。使用具有内H-2重组体的小鼠来绘制H-2复合物的I-A区域中的T-巨噬细胞相互作用基因座（以前称为聚-D，L-ala-聚-L-lys。还使用澄清剂排除H-2复合物的K、I-B或I-C、SS-Slp或D区的另一个T-巨噬细胞基因座的存在。在H-2-k、d、B、q、s基因型小鼠以及H-2重组体中，显示了辅助细胞诱导中T-巨噬细胞相互作用的遗传限制。讨论了这种遗传限制的可能机制和意义。
Helper cell induction to nonparticle antigens in vitro requires the cooperation of T cells and macrophages, but does not occur if the macrophages are allogenic. The reasons for this were investigated. Malfunction of allogenic macrophages was excluded by cultures with their syngenic T cells; suppressor cell induction was excluded by admixture experiments. Thus, T cells and macrophages only cooperated if they were genetically similar. The genetic locus (loci) involved was mapped. Using congenic lines differing only at the H-2 complex, the genetic control of T-macrophage interaction was localized in the H- 2 region. Mice with intra H-2 recombinants were used to map the T- macrophage interaction locus in the I-A region of the H-2 complex (formerly known as poly-D, L-ala-poly-L-lys. Recombinants were also used to exclude the presence of another T-macrophage locus either the K, I-B, or I-C, SS-Slp, or D regions of the H-2 complex. Genetic restrictions for T-macrophage interaction in helper cell induction was shown in mice of the H-2-k, d, b, q, s genotypes as well as in H-2 recombinants. The possible mechanisms and significance of this genetic restriction are discussed.